HitPredict

Interactor 1: DHX8 (Q14562)
ATP-dependent RNA helicase DHX8 (EC 3.6.4.13) (DEAH box protein 8) (RNA helicase HRH1)
Gene: DHX8

Interactor 2: SYF1 (Q9HCS7)
Pre-mRNA-splicing factor SYF1 (Protein HCNP) (XPA-binding protein 2)
Gene: XAB2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.895

Property	Details
Experiments	26186194, 28514442, 34079125, 33961781
Identification Methods	affinity chromatography technology, proximity labelling technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	DEAD~TPR_8
Common GO terms	GO:0000398 - mRNA splicing, via spliceosome GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0071007 - U2-type catalytic step 2 spliceosome GO:0071013 - catalytic step 2 spliceosome
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 1220 Hs			1 855
+	Hs
		224847 DHX16_HUMAN (319-1206): 0: 789: 43.93		SYF1_HUMAN (1-855): 0: 602: 100.00
+	Hs
		1055473 DHX8_HUMAN (11-1220): 0: 1147: 100.00		CRNL1_HUMAN (166-822): 5.62e-47: 182: 14.51
+	Hs
		1055399 DHX8_HUMAN (11-1220): 0: 1147: 100.00		CTR9_HUMAN (44-847): 4.01e-41: 165: 10.93
+	Hs
		300141 DHX8_HUMAN (11-1220): 0: 1147: 100.00		PRP6_HUMAN (2-353): 4.69e-26: 117: 12.92
+	Sc
		622340 PRP22_YEAST (249-1220): 0: 830: 47.21		SYF1_YEAST (19-733): 1.86e-87: 298: 22.31
+	Sc
		589452 PRP2_YEAST (349-1182): 0: 829: 38.12		SYF1_YEAST (19-733): 1.86e-87: 298: 22.31
+	Sc
		554246 PRP16_YEAST (292-1220): 0: 744: 36.88		SYF1_YEAST (19-733): 1.86e-87: 298: 22.31
+	Hs
		1160938 DHX40_HUMAN (566-1196): 0: 804: 42.92		CTR9_HUMAN (44-847): 4.01e-41: 165: 10.93
+	Sc
		622351 PRP22_YEAST (249-1220): 0: 830: 47.21		CLF1_YEAST (3-250): 5.35e-11: 68.5: 12.06
+	Sc
		589465 PRP2_YEAST (349-1182): 0: 829: 38.12		CLF1_YEAST (3-250): 5.35e-11: 68.5: 12.06
+	Sp
		194928 DHX15_SCHPO (494-1209): 0: 760: 47.07		CLF1_SCHPO (471-778): 7.87e-33: 137: 13.61
+	Hs
		1057967 DHX40_HUMAN (566-1196): 0: 804: 42.92		RRP5_HUMAN (11-347): 9.5e-18: 90.8: 11.26
+	Hs
		174828 DHX9_HUMAN (299-1177): 0: 674: 23.24		OGT1_HUMAN (7-468): 1.59e-37: 154: 13.55
+	Sc
		859429 PRP43_YEAST (479-1207): 0: 753: 46.40		RRP5_YEAST (507-723): 1.31e-11: 70.9: 15.46
+	Sc
		859463 PRP43_YEAST (479-1207): 0: 753: 46.40		CLF1_YEAST (3-250): 5.35e-11: 68.5: 12.06
+	Sc
		554256 PRP16_YEAST (292-1220): 0: 744: 36.88		CLF1_YEAST (3-250): 5.35e-11: 68.5: 12.06
+	Hs
		994172 DHX9_HUMAN (299-1177): 0: 674: 23.24		SART3_HUMAN (18-578): 1.7e-31: 134: 12.80