HitPredict

Interactor 1: ZNRF1 (Q8ND25)
E3 ubiquitin-protein ligase ZNRF1 (EC 2.3.2.27) (Nerve injury-induced gene 283 protein) (RING-type E3 ubiquitin transferase ZNRF1) (Zinc/RING finger protein 1)
Gene: ZNRF1

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.81	1.00	0.904

Property	Details
Experiments	32296183, 19690564, 19549727, unassigned1304
Identification Methods	two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 227 Hs			1 147
+	Hs
		849373 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919790 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895849 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1242075 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1242072 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851849 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896495 ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		1243628 Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		849374 Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919791 Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895850 Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1245518 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		849375 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919793 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895853 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1245511 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1245509 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851850 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896497 Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		896498 ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53