HitPredict

Interactor 1: SPK1 (Q8SAB7)
Guanine nucleotide exchange factor SPIKE 1
Gene: SPK1

Interactor 2: RAC6 (Q9SBJ6)
Rac-like GTP-binding protein ARAC6 (GTPase protein ROP5)
Gene: ARAC6

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.781

Property	Details
Experiments	17267444, 18308939
Identification Methods	two hybrid, pull down
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	DHR-2_Lobe_B~Ras, DHR-2_Lobe_C~Ras
Common GO terms	GO:0005634 - nucleus GO:0007264 - small GTPase-mediated signal transduction GO:0008360 - regulation of cell shape
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1830 At			1 197
+	At
		287603 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC10_ARATH (2-180): 3.07e-80: 239: 80.45
+	At
		1105105 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC1_ARATH (1-197): 3.13e-80: 238: 98.48
+	At
		1105136 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC4_ARATH (1-178): 3.37e-80: 238: 89.89
+	At
		1248269 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC9_ARATH (7-197): 7.37e-80: 238: 75.39
+	At
		1105166 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC5_ARATH (1-178): 2.55e-77: 231: 92.70
+	At
		1248268 SPK1_ARATH (1-1830): 0: 2088: 100.00		RAC8_ARATH (2-180): 9.92e-77: 230: 81.01
+	Hs
		925332 DOC11_HUMAN (77-1827): 0: 1614: 21.33		RAC1_HUMAN (7-197): 8.96e-81: 240: 61.14
+	Hs
		892410 DOC11_HUMAN (77-1827): 0: 1614: 21.33		RAC3_HUMAN (7-178): 2.4e-79: 236: 63.22
+	Hs
		545421 DOC11_HUMAN (77-1827): 0: 1614: 21.33		RAC2_HUMAN (7-197): 2.55e-77: 231: 60.10
+	Hs
		1127531 DOC11_HUMAN (77-1827): 0: 1614: 21.33		RHOJ_HUMAN (5-197): 1.15e-70: 215: 51.28
+	Hs
		564557 DOC11_HUMAN (77-1827): 0: 1614: 21.33		RHOQ_HUMAN (7-187): 1.58e-69: 212: 54.10
+	Hs
		925588 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RAC1_HUMAN (7-197): 8.96e-81: 240: 61.14
+	Hs
		892573 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RAC3_HUMAN (7-178): 2.4e-79: 236: 63.22
+	Hs
		545596 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RAC2_HUMAN (7-197): 2.55e-77: 231: 60.10
+	Hs
		951301 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RHOG_HUMAN (6-197): 1.45e-76: 229: 54.64
+	Hs
		1317682 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RHOJ_HUMAN (5-197): 1.15e-70: 215: 51.28
+	Hs
		564671 DOCK9_HUMAN (46-1827): 0: 1537: 19.31		RHOQ_HUMAN (7-187): 1.58e-69: 212: 54.10
+	Hs
		925518 DOCK6_HUMAN (16-979): 0: 755: 20.07		RAC1_HUMAN (7-197): 8.96e-81: 240: 61.14
+	Hs
		925526 DOCK7_HUMAN (14-731): 0: 656: 21.52		RAC1_HUMAN (7-197): 8.96e-81: 240: 61.14
+	Hs
		925448 DOCK8_HUMAN (14-731): 0: 602: 20.24		RAC1_HUMAN (7-197): 8.96e-81: 240: 61.14