HitPredict

Interactor 1: WNK3 (Q9BYP7)
Serine/threonine-protein kinase WNK3 (EC 2.7.11.1) (Protein kinase lysine-deficient 3) (Protein kinase with no lysine 3)
Gene: WNK3

Interactor 2: T22D4 (Q9Y3Q8)
TSC22 domain family protein 4 (TSC22-related-inducible leucine zipper protein 2)
Gene: TSC22D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 1800 Hs			1 395
+	Hs
		256827 WNK3_HUMAN (1-1800): 0: 1277: 100.00		T22D2_HUMAN (23-388): 4.37e-28: 118: 27.69
+	Hs
		1377724 WNK2_HUMAN (1-719): 2.5e-112: 400: 49.73		T22D4_HUMAN (1-395): 1.5e-63: 210: 100.00
+	Hs
		1330751 WNK1_HUMAN (27-1637): 1.09e-101: 366: 26.62		T22D4_HUMAN (1-395): 1.5e-63: 210: 100.00
+	Rn
		914592 AKT3_RAT (52-472): 7.32e-131: 419: 17.96		T22D1_RAT (28-394): 4.07e-36: 142: 27.59
+	Hs
		1077338 WNK1_HUMAN (27-1637): 1.09e-101: 366: 26.62		T22D1_HUMAN (28-394): 4.95e-38: 148: 26.81
+	Hs
		1158052 MAST2_HUMAN (119-1324): 1.37e-126: 441: 13.67		TITIN_HUMAN (5-287): 1.18e-10: 65.7: 11.75
+	Hs
		1345645 NRBP2_HUMAN (141-413): 3.69e-31: 132: 31.62		T22D4_HUMAN (1-395): 1.5e-63: 210: 100.00
+	Hs
		1355633 NRBP_HUMAN (142-413): 1.69e-30: 130: 33.92		T22D4_HUMAN (1-395): 1.5e-63: 210: 100.00
+	Hs
		1217314 NRBP_HUMAN (142-413): 1.69e-30: 130: 33.92		Q8IV54_HUMAN (1-310): 5.68e-44: 156: 99.68
+	Hs
		1077348 NRBP2_HUMAN (141-413): 3.69e-31: 132: 31.62		T22D1_HUMAN (28-394): 4.95e-38: 148: 26.81
+	Hs
		1077357 NRBP_HUMAN (142-413): 1.69e-30: 130: 33.92		T22D1_HUMAN (28-394): 4.95e-38: 148: 26.81
+	Hs
		256831 NRBP2_HUMAN (141-413): 3.69e-31: 132: 31.62		T22D2_HUMAN (23-388): 4.37e-28: 118: 27.69
+	Hs
		256836 NRBP_HUMAN (142-413): 1.69e-30: 130: 33.92		T22D2_HUMAN (23-388): 4.37e-28: 118: 27.69
+	Hs
		1137771 OBSCN_HUMAN (110-421): 9.24e-45: 181: 20.63		TITIN_HUMAN (5-287): 1.18e-10: 65.7: 11.75
+	Hs
		1304681 NRBP2_HUMAN (141-413): 3.69e-31: 132: 31.62		T22D3_HUMAN (276-395): 2.33e-27: 107: 49.59
+	Hs
		1304682 NRBP_HUMAN (142-413): 1.69e-30: 130: 33.92		T22D3_HUMAN (276-395): 2.33e-27: 107: 49.59
+	Dm
		1098027 NRBP_DROME (130-413): 3.08e-27: 121: 32.23		BUN2_DROME (139-390): 4.04e-11: 66.9: 26.92