HitPredict

Interactor 1: MAD2 (O14417)
Mitotic spindle checkpoint component mad2
Gene: MAD2

Interactor 2: MAD3 (O59767)
Mitotic spindle checkpoint component mad3
Gene: MAD3

Species: Schizosaccharomyces pombe 972h-

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	0.60	0.691

Property	Details
Experiments	28366743, 11909965, 12676091, 18556659
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000776 - kinetochore GO:0007094 - mitotic spindle assembly checkpoint signaling GO:0010997 - anaphase-promoting complex binding GO:0033597 - mitotic checkpoint complex GO:0045841 - negative regulation of mitotic metaphase/anaphase transition and 1 others more... GO:0051301 - cell division
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 203 Sp			1 310
+	At
		187358 MAD2_ARATH (5-202): 8.73e-90: 263: 41.92		BUBR1_ARATH (15-288): 1.6e-96: 292: 27.00
+	Hs
		236921 MD2L1_HUMAN (7-200): 1.79e-87: 258: 46.91		BUB1B_HUMAN (14-291): 1.57e-87: 284: 26.21
+	Dm
		51409 Q9VRQ2_DROME (2-202): 5.96e-86: 254: 38.54		A1Z6I7_DROME (15-271): 5.11e-81: 269: 24.90
+	Sc
		778483 MAD2_YEAST (8-200): 2.96e-79: 236: 48.21		MAD3_YEAST (3-275): 2.58e-80: 254: 28.43
+	Hs
		211011 MD2L1_HUMAN (7-200): 1.79e-87: 258: 46.91		BUB1_HUMAN (48-270): 1.64e-67: 229: 27.35
+	Sp
		145599 MAD2_SCHPO (1-203): 2.15e-91: 267: 100.00		BUB1_SCHPO (39-276): 3.59e-47: 171: 21.12
+	Sc
		778482 MAD2_YEAST (8-200): 2.96e-79: 236: 48.21		BUB1_YEAST (18-225): 5.24e-50: 179: 24.19