HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: UB2D2 (P62837)
Ubiquitin-conjugating enzyme E2 D2 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D2) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D2) (Ubiquitin carrier protein D2) (Ubiquitin-conjugating enzyme E2(17)KB 2) (Ubiquitin-conjugating enzyme E2-17 kDa 2) (Ubiquitin-protein ligase D2) (p53-regulated ubiquitin-conjugating enzyme 1)
Gene: UBE2D2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.967

Property	Details
Experiments	19549727, 21143188, 25207814, 25278022, 32296183, 22613722, unassigned1304
Identification Methods	two hybrid, pull down, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 667 Hs			1 147
+	Hs
		175730 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20
+	Hs
		175653 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12
+	Hs
		175658 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	Hs
		175710 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E2_HUMAN (4-146): 5.85e-75: 223: 65.73
+	Hs
		175703 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E3_HUMAN (4-146): 3.05e-74: 221: 66.43
+	Hs
		1151762 Q6GX22_HUMAN (1-665): 1.26e-170: 505: 76.24		UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20
+	Hs
		175654 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E1_HUMAN (4-146): 8.81e-73: 217: 65.73
+	Hs
		175660 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22
+	Hs
		1356055 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20
+	Hs
		849480 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12
+	Hs
		919896 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D2_HUMAN (1-147): 9.5e-83: 241: 100.00
+	Hs
		895945 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	Hs
		1297378 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E2_HUMAN (4-146): 5.85e-75: 223: 65.73
+	Hs
		1282363 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E3_HUMAN (4-146): 3.05e-74: 221: 66.43
+	Hs
		896464 Q6GX22_HUMAN (1-665): 1.26e-170: 505: 76.24		UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22
+	Hs
		851905 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E1_HUMAN (4-146): 8.81e-73: 217: 65.73
+	Hs
		896562 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22
+	Hs
		849330 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12
+	Hs
		895794 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	Hs
		896442 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22