HitPredict

Interactor 1: EFS (O43281)
Embryonal Fyn-associated substrate (hEFS) (Cas scaffolding protein family member 3)
Gene: EFS

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.57	0.60	0.586

Property	Details
Experiments	26871637, 17474147
Identification Methods	two hybrid, peptide array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0016477 - cell migration GO:0019904 - protein domain specific binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 561 Hs			1 377
+	Hs
		199489 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		557691 CASL_HUMAN (6-336): 2.64e-31: 131: 24.93		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Mm
		222878 ACK1_MOUSE (11-484): 7.98e-29: 124: 15.31		NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94
+	Hs
		557740 MINK1_HUMAN (48-378): 5.94e-26: 115: 14.67		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Hs
		209112 MINK1_HUMAN (48-378): 5.94e-26: 115: 14.67		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		209039 ACK1_HUMAN (9-484): 1.17e-23: 107: 14.85		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		91474 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		E7ERP6_HUMAN (1-76): 3.94e-36: 128: 78.95
+	Hs
		3145 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		A0A0S2Z4E4_HUMAN (1-181): 3.34e-26: 107: 74.87
+	Hs
		199493 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26
+	Hs
		199505 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Hs
		199497 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		3132 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		A0A0S2Z4D7_HUMAN (105-253): 4.97e-18: 82.5: 20.40
+	Hs
		199500 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32
+	Hs
		199510 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		199498 EFS_HUMAN (1-561): 3.37e-71: 239: 100.00		YES_HUMAN (112-218): 4.18e-11: 66.1: 19.27
+	Mm
		403448 EFS_MOUSE (1-561): 2.45e-61: 213: 79.50		SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32
+	Hs
		412193 CASL_HUMAN (6-336): 2.64e-31: 131: 24.93		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		412152 ACK1_HUMAN (9-484): 1.17e-23: 107: 14.85		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		924703 MINK1_HUMAN (48-378): 5.94e-26: 115: 14.67		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		433331 ACK1_HUMAN (9-484): 1.17e-23: 107: 14.85		YES_HUMAN (112-218): 4.18e-11: 66.1: 19.27