HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: KIT (P10721)
Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Piebald trait protein) (PBT) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (p145 c-kit) (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (CD antigen CD117)
Gene: KIT

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.692

Property	Details
Experiments	24728074
Identification Methods	pull down, fluorescence polarization spectroscopy
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1, PK_Tyr_Ser-Thr~SH3_9
Common GO terms	GO:0007165 - signal transduction GO:0030032 - lamellipodium assembly
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 976
+	Hs
		499225 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		KIT_HUMAN (1-976): 0: 807: 100.00
+	Hs
		499264 GRAP_HUMAN (5-169): 7.3e-28: 110: 15.74		KIT_HUMAN (1-976): 0: 807: 100.00
+	Hs
		499235 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		KIT_HUMAN (1-976): 0: 807: 100.00
+	Hs
		208979 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		PGFRB_HUMAN (30-954): 0: 591: 30.62
+	Mm
		403533 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		KIT_MOUSE (1-975): 0: 773: 79.63
+	Hs
		452344 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		PGFRB_HUMAN (30-954): 0: 591: 30.62
+	Mm
		404009 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		PGFRB_MOUSE (14-974): 0: 597: 29.51
+	Hs
		452361 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		PGFRB_HUMAN (30-954): 0: 591: 30.62
+	Hs
		208993 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		FGFR4_HUMAN (4-224): 3.99e-23: 107: 13.21
+	Hs
		208983 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		FGFR1_HUMAN (4-235): 6.88e-22: 104: 13.25
+	Hs
		557606 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FGFR4_HUMAN (4-224): 3.99e-23: 107: 13.21
+	Hs
		507832 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FGFR1_HUMAN (4-235): 6.88e-22: 104: 13.25
+	Hs
		557625 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		VGFR2_HUMAN (3-106): 1.62e-13: 77.5: 14.04
+	Hs
		557624 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		VGFR3_HUMAN (3-111): 3.96e-11: 69.4: 13.93
+	Hs
		601605 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		FGFR2_HUMAN (8-323): 1.59e-55: 209: 13.10
+	Hs
		608159 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		FGFR4_HUMAN (4-224): 3.99e-23: 107: 13.21
+	Hs
		508233 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		FGFR1_HUMAN (4-235): 6.88e-22: 104: 13.25
+	Hs
		507870 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		FGFR1_HUMAN (4-235): 6.88e-22: 104: 13.25
+	Mm
		452179 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		CSF1R_MOUSE (6-304): 6.2e-18: 91.3: 13.53
+	Hs
		426986 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		CSF1R_HUMAN (209-511): 1.03e-12: 74.4: 14.10