HitPredict

Interactor 1: RPB4 (O74825)
DNA-directed RNA polymerase II subunit rpb4 (RNA polymerase II subunit B4)
Gene: RPB4

Interactor 2: RPB1 (P36594)
DNA-directed RNA polymerase II subunit rpb1 (RNA polymerase II subunit 1) (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit)
Gene: RPB1

Species: Schizosaccharomyces pombe 972h-

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.54	1.00	0.737

Property	Details
Experiments	15743411, 14534314
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	RNA_pol_Rpb1_1~RNA_pol_Rpb4, RNA_pol_Rpb1_R~RNA_pol_Rpb4
Common GO terms	GO:0005634 - nucleus GO:0005665 - RNA polymerase II, core complex GO:0006366 - transcription by RNA polymerase II GO:0006367 - transcription initiation at RNA polymerase II promoter GO:0016591 - RNA polymerase II, holoenzyme
Homologous interactions	8

Homologous interactions

Color coding for hit scores

	1 135 Sp			1 1752
+	Sc
		375268 RPB4_YEAST (55-133): 2.65e-21: 86.7: 47.50		RPB1_YEAST (1-1564): 0: 1617: 61.06
+	Hs
		155964 RPB4_HUMAN (6-134): 1.19e-45: 146: 40.77		RPC1_HUMAN (1-1460): 0: 1302: 33.85
+	Hs
		181375 RPB4_HUMAN (6-134): 1.19e-45: 146: 40.77		RPA1_HUMAN (919-1452): 1.39e-40: 167: 14.82
+	At
		1125366 NRPD4_ARATH (20-134): 9.68e-37: 126: 21.85		NRPE1_ARATH (843-1455): 6e-34: 145: 14.90
+	At
		1150031 NRPD4_ARATH (20-134): 9.68e-37: 126: 21.85		NRPD1_ARATH (844-1491): 4.34e-33: 142: 15.89
+	Sc
		501314 RPB4_YEAST (55-133): 2.65e-21: 86.7: 47.50		RPA1_YEAST (9-199): 4.72e-35: 149: 25.13
+	Bt
		102290 RPB4_BOVIN (6-134): 1.4e-45: 146: 40.77		RPB1_BOVIN (1489-1699): 7.68e-16: 86.1: 52.56
+	Hs
		181390 RPB4_HUMAN (6-134): 1.19e-45: 146: 40.77		RPB1_HUMAN (1489-1699): 7.68e-16: 86.1: 52.56