HitPredict

Interactor 1: PIAS1 (O75925)
E3 SUMO-protein ligase PIAS1 (EC 2.3.2.-) (DEAD/H box-binding protein 1) (E3 SUMO-protein transferase PIAS1) (Gu-binding protein) (GBP) (Protein inhibitor of activated STAT protein 1) (RNA helicase II-binding protein)
Gene: PIAS1

Interactor 2: SMAD1 (Q15797)
Mothers against decapentaplegic homolog 1 (MAD homolog 1) (Mothers against DPP homolog 1) (JV4-1) (Mad-related protein 1) (SMAD family member 1) (SMAD 1) (Smad1) (hSMAD1) (Transforming growth factor-beta-signaling protein 1) (BSP-1)
Gene: SMAD1

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.43	0.60	0.508

Property	Details
Experiments	15231748
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0006357 - regulation of transcription by RNA polymerase II GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 651 Hs			1 465
+	Hs
		278075 PIAS1_HUMAN (1-651): 0: 874: 100.00		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		950349 PIAS3_HUMAN (1-651): 0: 740: 56.88		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1078712 PIAS3_HUMAN (1-651): 0: 740: 56.88		SMAD2_HUMAN (3-465): 0: 541: 55.14
+	Hs
		1078729 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD1_HUMAN (1-465): 0: 658: 100.00
+	Hs
		278264 PIAS2_HUMAN (1-566): 0: 633: 58.97		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1035653 PIAS3_HUMAN (1-651): 0: 740: 56.88		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		950220 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1078585 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD2_HUMAN (3-465): 0: 541: 55.14
+	Hs
		278278 PIAS2_HUMAN (1-566): 0: 633: 58.97		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		206981 PIAS3_HUMAN (1-651): 0: 740: 56.88		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		1035500 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		167316 PIAS1_HUMAN (1-651): 0: 874: 100.00		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46
+	Hs
		206968 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		950322 ZMIZ1_HUMAN (65-441): 7.58e-76: 264: 20.21		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1035630 ZMIZ1_HUMAN (65-441): 7.58e-76: 264: 20.21		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		167374 PIAS4_HUMAN (2-423): 0: 550: 51.42		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46