HitPredict

Interactor 1: PAK4 (O96013)
Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (p21-activated kinase 4) (PAK-4)
Gene: PAK4

Interactor 2: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.85	0.60	0.714

Property	Details
Experiments	35271311, 32707033, 36931259, 17979178, 29100370
Identification Methods	affinity chromatography technology, proximity labelling technology, anti tag coimmunoprecipitation, pull down, cross-linking study
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005925 - focal adhesion GO:0007165 - signal transduction GO:0035556 - intracellular signal transduction
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 591 Hs			1 255
+	Hs
		910063 PAK6_HUMAN (1-591): 2.91e-166: 491: 45.72		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		909811 DCLK2_HUMAN (31-591): 2.1e-132: 406: 17.65		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Mm
		910275 DCLK2_MOUSE (31-591): 2.24e-132: 406: 17.13		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Mm
		910345 DCLK1_MOUSE (31-591): 1.3e-130: 401: 18.74		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Hs
		909621 MYLK3_HUMAN (95-580): 2.48e-128: 397: 23.48		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Sc
		713000 STE20_YEAST (11-591): 6.96e-143: 439: 31.62		BMH2_YEAST (4-242): 1.14e-134: 382: 77.08
+	Sc
		665025 STE20_YEAST (11-591): 6.96e-143: 439: 31.62		BMH1_YEAST (2-243): 2.43e-134: 381: 75.72
+	At
		965291 CDPK1_ARATH (182-591): 2.62e-130: 396: 24.35		14332_ARATH (4-255): 1.05e-147: 414: 74.41
+	At
		965360 CIPKC_ARATH (302-577): 2.61e-131: 394: 25.18		14332_ARATH (4-255): 1.05e-147: 414: 74.41
+	At
		788672 CIPKC_ARATH (302-577): 2.61e-131: 394: 25.18		14333_ARATH (1-253): 3.45e-147: 413: 74.12
+	At
		1280891 CIPKC_ARATH (302-577): 2.61e-131: 394: 25.18		14337_ARATH (2-255): 7.73e-147: 412: 71.21
+	At
		802001 CDPK1_ARATH (182-591): 2.62e-130: 396: 24.35		14334_ARATH (4-255): 2.51e-145: 408: 73.62
+	Hs
		909512 STK3_HUMAN (289-588): 1.76e-123: 374: 35.43		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		909502 STK4_HUMAN (298-588): 1.44e-122: 372: 35.50		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	At
		788694 CDPK1_ARATH (182-591): 2.62e-130: 396: 24.35		14335_ARATH (2-255): 3.04e-141: 398: 68.75
+	Hs
		910114 PAK5_HUMAN (1-323): 8.93e-40: 156: 27.68		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00