HitPredict

Interactor 1: RPOD (P00579)
RNA polymerase sigma factor RpoD (Sigma-70)
Gene: RPOD

Interactor 2: RPOB (P0A8V2)
DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)
Gene: RPOB

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.84 1.00 0.919

The method score is based on:

Property Details
Experiments 16606699, 28524877, 19366670, 22135460, 15690043
Identification Methods affinity chromatography technology, cross-linking study, lambda repressor two hybrid, tandem affinity purification, pull down
Interaction Type physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains RNA_pol_Rpb2_6~Sigma70_ner, RNA_pol_Rpb2_1~Sigma70_r1_1, RNA_pol_Rpb2_1~Sigma70_r1_2, RNA_pol_Rpb2_1~Sigma70_r2, RNA_pol_Rpb2_6~Sigma70_r2, RNA_pol_Rpb2_7~Sigma70_r2, RNA_pol_Rpb2_1~Sigma70_r3, RNA_pol_Rpb2_3~Sigma70_r3, RNA_pol_Rpb2_6~Sigma70_r3, RNA_pol_Rpb2_7~Sigma70_r3, RNA_pol_Rpb2_6~Sigma70_r4
Common GO terms GO:0000345 - cytosolic DNA-directed RNA polymerase complex
GO:0003677 - DNA binding
GO:0005829 - cytosol
GO:0006352 - DNA-templated transcription initiation
GO:0009408 - response to heat
and 1 others

more...
Homologous interactions 12

Homologous interactions

Color coding for hit scores

1 613
Ec

1 1342
+ Nv
+ Hp
+ Ec
+ Ec
+ Ec
+ Hp
+ Mp
+ Bs
+ Ss
+ Ss
+ Ss
+ Bs

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Last updated: 11Aug2025