HitPredict

Interactor 1: A4 (P05067)
Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid A4 protein homolog) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta (A4) precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
Gene: APP

Interactor 2: NUMB (P49757)
Protein numb homolog (h-Numb) (Protein S171)
Gene: NUMB

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	21832049, 32814053
Identification Methods	pull down, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	APP_amyloid~PID
Common GO terms	GO:0005737 - cytoplasm GO:0005769 - early endosome GO:0005886 - plasma membrane GO:0005905 - clathrin-coated pit GO:0007409 - axonogenesis and 1 others more... GO:0045177 - apical part of cell
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 770 Hs			1 651
+	Mm
		517715 A4_MOUSE (1-770): 0: 1016: 96.62		NUMB_MOUSE (1-651): 8.07e-101: 323: 92.96
+	Hs
		395052 A4_HUMAN (19-770): 0: 999: 100.00		ANS1B_HUMAN (7-215): 9.71e-51: 192: 19.05
+	Hs
		394888 A4_HUMAN (19-770): 0: 999: 100.00		ARH_HUMAN (4-293): 1.38e-52: 185: 19.67
+	Mm
		517621 A4_MOUSE (1-770): 0: 1016: 96.62		DAB2_MOUSE (5-598): 3.19e-36: 147: 13.49
+	Mm
		517618 A4_MOUSE (1-770): 0: 1016: 96.62		DAB1_MOUSE (31-622): 6.85e-35: 141: 15.66
+	Hs
		253522 A4_HUMAN (19-770): 0: 999: 100.00		CAPON_HUMAN (8-286): 2.51e-35: 141: 17.11
+	Hs
		269118 A4_HUMAN (19-770): 0: 999: 100.00		DAB1_HUMAN (31-622): 1.27e-34: 141: 15.66
+	Hs
		394535 A4_HUMAN (19-770): 0: 999: 100.00		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Hs
		269140 APLP2_HUMAN (3-769): 0: 858: 49.17		DAB1_HUMAN (31-622): 1.27e-34: 141: 15.66
+	Hs
		957169 APLP2_HUMAN (3-769): 0: 858: 49.17		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Mm
		956226 APLP2_MOUSE (3-769): 0: 753: 44.32		DAB1_MOUSE (31-622): 6.85e-35: 141: 15.66
+	Hs
		269127 APLP1_HUMAN (6-194): 9.65e-70: 243: 45.79		DAB1_HUMAN (31-622): 1.27e-34: 141: 15.66
+	Mm
		956224 APLP1_MOUSE (5-193): 1.54e-68: 240: 44.74		DAB1_MOUSE (31-622): 6.85e-35: 141: 15.66
+	Hs
		849925 APLP1_HUMAN (6-194): 9.65e-70: 243: 45.79		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Hs
		253541 TFPI2_HUMAN (210-337): 4.79e-14: 74.2: 20.14		CAPON_HUMAN (8-286): 2.51e-35: 141: 17.11