HitPredict

Interactor 1: CAN1 (P07384)
Calpain-1 catalytic subunit (EC 3.4.22.52) (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Cell proliferation-inducing gene 30 protein) (Micromolar-calpain) (muCANP)
Gene: CAPN1

Interactor 2: CPNS2 (Q96L46)
Calpain small subunit 2 (CSS2) (Calcium-dependent protease small subunit 2)
Gene: CAPNS2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	28514442, 33961781, 26186194
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Calpain_III~EF-hand_8, EF-hand_8~EF-hand_8
Common GO terms	GO:0004198 - calcium-dependent cysteine-type endopeptidase activity GO:0005509 - calcium ion binding GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0110158 - calpain complex
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 714 Hs			1 248
+	Hs
		385827 CAN1_HUMAN (1-714): 0: 950: 100.00		CPNS1_HUMAN (40-248): 3.17e-64: 202: 72.25
+	Hs
		427830 CAN1_HUMAN (1-714): 0: 950: 100.00		CAN1_HUMAN (26-248): 1.42e-59: 201: 41.07
+	Hs
		427838 CAN1_HUMAN (1-714): 0: 950: 100.00		CAN2_HUMAN (26-243): 4.35e-54: 186: 38.07
+	Hs
		385837 CAN2_HUMAN (13-710): 0: 917: 62.75		CPNS1_HUMAN (40-248): 3.17e-64: 202: 72.25
+	Hs
		62711 CAN1_HUMAN (1-714): 0: 950: 100.00		CAN8_HUMAN (15-246): 1.91e-47: 168: 34.34
+	Rn
		989740 CAN2_RAT (13-710): 0: 903: 61.89		CPNS1_RAT (40-248): 8.22e-62: 196: 69.86
+	Hs
		595808 CAN3_HUMAN (10-714): 0: 853: 46.18		CAN3_HUMAN (43-248): 8.56e-58: 198: 46.60
+	Dm
		1373832 CANB_DROME (41-714): 0: 806: 43.37		CANB_DROME (55-248): 1.08e-55: 193: 32.14
+	Dm
		1373833 CANB_DROME (41-714): 0: 806: 43.37		CANC_DROME (58-243): 2.78e-53: 184: 25.27
+	Dm
		1014697 CANB_DROME (41-714): 0: 806: 43.37		CANA_DROME (107-248): 2.29e-45: 163: 38.03
+	Dm
		1014698 CANA_DROME (40-714): 0: 754: 39.06		CANC_DROME (58-243): 2.78e-53: 184: 25.27
+	Dm
		906742 CANC_DROME (38-712): 0: 565: 29.40		CALM_DROME (18-151): 4.46e-16: 74.6: 14.29
+	Dm
		647734 CAND_DROME (20-507): 4.41e-136: 439: 24.90		CANA_DROME (107-248): 2.29e-45: 163: 38.03
+	Hs
		659615 GRAN_HUMAN (534-712): 5.09e-64: 213: 30.17		GRAN_HUMAN (37-246): 9.93e-55: 177: 29.55
+	Hs
		659616 GRAN_HUMAN (534-712): 5.09e-64: 213: 30.17		SORCN_HUMAN (73-246): 6.05e-46: 153: 29.31