HitPredict

Interactor 1: UBC (P0CG48)
Polyubiquitin-C [Cleaved into: Ubiquitin]
Gene: UBC

Interactor 2: NEDD4 (P46934)
E3 ubiquitin-protein ligase NEDD4 (EC 2.3.2.26) (Cell proliferation-inducing gene 53 protein) (HECT-type E3 ubiquitin transferase NEDD4) (Neural precursor cell expressed developmentally down-regulated protein 4) (NEDD-4)
Gene: NEDD4

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.965

Property	Details
Experiments	25438670, 26371805, 29964046, 21399620, 23287719, 23644597, 19343052
Identification Methods	x-ray crystallography, enzymatic study, pull down, unspecified method
Interaction Type	association, direct interaction

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016567 - protein ubiquitination GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 685 Hs			1 1319
+	Hs
		476229 UBC_HUMAN (1-685): 0: 846: 100.00		NED4L_HUMAN (517-1319): 0: 934: 62.26
+	Hs
		475963 UBC_HUMAN (1-685): 0: 846: 100.00		HECW1_HUMAN (475-1317): 0: 726: 31.48
+	Hs
		475984 UBC_HUMAN (1-685): 0: 846: 100.00		HUWE1_HUMAN (799-1317): 6.4e-169: 562: 35.32
+	Dm
		89879 UBIQP_DROME (1-610): 0: 752: 99.84		D5SHR3_DROME (898-1316): 7.77e-180: 540: 45.99
+	Dm
		1376481 Q9W418_DROME (229-685): 0: 557: 99.78		Q9W325_DROME (899-1316): 2.66e-179: 538: 46.10
+	Hs
		476350 UBC_HUMAN (1-685): 0: 846: 100.00		WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86
+	Hs
		125113 UBC_HUMAN (1-685): 0: 846: 100.00		WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91
+	Sp
		143368 UBI4P_SCHPO (305-685): 1.76e-151: 445: 95.80		PTR1_SCHPO (820-1316): 7.49e-161: 538: 33.07
+	Hs
		476205 UBC_HUMAN (1-685): 0: 846: 100.00		ITCH_HUMAN (468-799): 2.73e-28: 125: 19.42
+	Hs
		476403 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF2_HUMAN (454-719): 3.19e-26: 118: 17.86
+	Hs
		476414 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF1_HUMAN (539-719): 7.11e-18: 91.8: 16.82
+	Sc
		477424 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		TOM1_YEAST (899-1317): 8.1e-155: 520: 40.42
+	Ce
		477557 UBIQ1_CAEEL (1-229): 5.97e-87: 292: 98.25		Q9GUP2_CAEEL (800-1316): 5.41e-158: 529: 33.96
+	At
		1091758 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL1_ARATH (837-1316): 5.48e-164: 547: 36.60
+	At
		1091762 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL2_ARATH (837-1316): 9.11e-162: 540: 35.60
+	Mm
		924239 RS27A_MOUSE (609-684): 9.1e-41: 147: 100.00		HUWE1_MOUSE (799-1317): 8.82e-169: 561: 35.25
+	Mm
		476858 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		HUWE1_MOUSE (799-1317): 8.82e-169: 561: 35.25
+	Sc
		477293 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		RSP5_YEAST (388-669): 1.92e-30: 132: 14.41
+	Hs
		924156 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86
+	Hs
		125347 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91