HitPredict

Interactor 1: UBC (P0CG48)
Polyubiquitin-C [Cleaved into: Ubiquitin]
Gene: UBC

Interactor 2: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.71	0.60	0.654

Property	Details
Experiments	31980649, 20936779, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005789 - endoplasmic reticulum membrane GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0070062 - extracellular exosome
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 685 Hs			1 192
+	Hs
		475625 UBC_HUMAN (1-685): 0: 846: 100.00		RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35
+	Hs
		475548 UBC_HUMAN (1-685): 0: 846: 100.00		CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31
+	Hs
		475558 UBC_HUMAN (1-685): 0: 846: 100.00		RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98
+	Hs
		923947 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00
+	Rn
		1144214 UBC_RAT (1-108): 1.27e-29: 127: 90.74		RAC1_RAT (1-192): 3.81e-86: 253: 100.00
+	Mm
		476750 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00
+	Hs
		900357 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98
+	Rn
		1104200 UBC_RAT (1-108): 1.27e-29: 127: 90.74		Q32PX6_RAT (1-192): 3.1e-79: 236: 71.35
+	Rn
		1144234 UBC_RAT (1-108): 1.27e-29: 127: 90.74		CDC42_RAT (1-192): 1.4e-78: 234: 70.31
+	Hs
		474987 UBB_HUMAN (1-77): 7.83e-26: 108: 98.70		RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00
+	Hs
		475188 UBB_HUMAN (1-77): 7.83e-26: 108: 98.70		RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64