HitPredict

Interactor 1: ARAF (P10398)
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Gene: ARAF

Interactor 2: 1433T (P27348)
14-3-3 protein theta (14-3-3 protein T-cell) (14-3-3 protein tau) (Protein HS1)
Gene: YWHAQ

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.965

Property	Details
Experiments	25852190, 29777862, 31980649, 32707033, 35271311, 36931259, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 606 Hs			1 245
+	Hs
		488645 ARAF_HUMAN (1-606): 0: 579: 100.00		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		488665 ARAF_HUMAN (1-606): 0: 579: 100.00		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		488590 ARAF_HUMAN (1-606): 0: 579: 100.00		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Hs
		110869 H7C560_HUMAN (3-604): 0: 547: 56.68		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		542849 BRAF_HUMAN (3-604): 0: 546: 56.68		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		110870 H7C560_HUMAN (3-604): 0: 547: 56.68		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		542820 BRAF_HUMAN (3-604): 0: 546: 56.68		1433T_HUMAN (1-245): 9.47e-142: 398: 100.00
+	Hs
		542857 BRAF_HUMAN (3-604): 0: 546: 56.68		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Gg
		982840 BRAF_CHICK (3-604): 0: 542: 56.20		1433T_CHICK (1-245): 3.09e-141: 397: 98.78
+	Gg
		982836 BRAF_CHICK (3-604): 0: 542: 56.20		1433Z_CHICK (1-245): 7.94e-141: 396: 79.18
+	Hs
		542827 BRAF_HUMAN (3-604): 0: 546: 56.68		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Gg
		982838 BRAF_CHICK (3-604): 0: 542: 56.20		1433B_CHICK (1-242): 6.39e-126: 358: 80.99
+	Hs
		488591 ARAF_HUMAN (1-606): 0: 579: 100.00		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Hs
		488691 ARAF_HUMAN (1-606): 0: 579: 100.00		1433F_HUMAN (2-242): 7.16e-106: 307: 71.43
+	Hs
		488639 ARAF_HUMAN (1-606): 0: 579: 100.00		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33
+	Hs
		542828 BRAF_HUMAN (3-604): 0: 546: 56.68		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Hs
		542868 BRAF_HUMAN (3-604): 0: 546: 56.68		1433F_HUMAN (2-242): 7.16e-106: 307: 71.43
+	Hs
		542847 BRAF_HUMAN (3-604): 0: 546: 56.68		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33
+	Gg
		982837 BRAF_CHICK (3-604): 0: 542: 56.20		Q5ZKJ2_CHICK (2-242): 5.28e-106: 308: 71.43
+	Gg
		982833 BRAF_CHICK (3-604): 0: 542: 56.20		1433G_CHICK (1-242): 2.55e-105: 306: 70.33