HitPredict

Interactor 1: HINT (P0ACE7)
Purine nucleoside phosphoramidase (EC 3.9.1.-) (Histidine triad nucleotide binding protein HinT) (HIT protein)
Gene: HINT

Interactor 2: HINT (P0ACE7)
Purine nucleoside phosphoramidase (EC 3.9.1.-) (Histidine triad nucleotide binding protein HinT) (HIT protein)
Gene: HINT

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	24561554
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	HIT~HIT
Common GO terms	GO:0000166 - nucleotide binding GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0042803 - protein homodimerization activity GO:0043530 - adenosine 5'-monophosphoramidase activity and 1 others more... GO:0055130 - D-alanine catabolic process
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 119 Ec			1 119
+	Hs
		1416525 HINT2_HUMAN (2-110): 5.35e-43: 140: 46.79		HINT2_HUMAN (2-110): 5.35e-43: 140: 46.79
+	Mm
		1423988 HINT2_MOUSE (2-110): 9.64e-43: 139: 47.71		HINT2_MOUSE (2-110): 9.64e-43: 139: 47.71
+	Ss
		1002053 YHIT_SYNY3 (1-111): 2.53e-41: 134: 49.55		YHIT_SYNY3 (1-111): 2.53e-41: 134: 49.55
+	Hs
		890181 HINT1_HUMAN (4-110): 1.3e-40: 132: 47.66		HINT1_HUMAN (4-110): 1.3e-40: 132: 47.66
+	Hs
		890224 HINT1_HUMAN (4-110): 1.3e-40: 132: 47.66		HINT3_HUMAN (2-111): 4.17e-29: 105: 23.01
+	Sc
		1046467 HNT1_YEAST (1-108): 1.43e-34: 118: 33.33		HNT1_YEAST (1-108): 1.43e-34: 118: 33.33
+	Mp
		1003561 YHIT_MYCPN (2-111): 2.27e-34: 117: 26.79		YHIT_MYCPN (2-111): 2.27e-34: 117: 26.79
+	Sp
		890240 APH1_SCHPO (1-111): 5.02e-33: 115: 23.42		APH1_SCHPO (1-111): 5.02e-33: 115: 23.42
+	Sc
		890238 HNT2_YEAST (1-111): 2.17e-32: 114: 19.64		HNT2_YEAST (1-111): 2.17e-32: 114: 19.64
+	Cj
		1077869 Q0PA00_CAMJE (1-110): 2.82e-33: 113: 38.18		Q0PA00_CAMJE (1-110): 2.82e-33: 113: 38.18
+	Sp
		314470 HNT1_SCHPO (4-107): 1.58e-32: 112: 26.92		HNT1_SCHPO (4-107): 1.58e-32: 112: 26.92
+	Ca
		1202757 HNT1_CANAL (3-108): 1.09e-31: 111: 29.46		HNT1_CANAL (3-108): 1.09e-31: 111: 29.46
+	Hp
		202546 O25440_HELPY (2-108): 7.75e-28: 101: 21.43		O25440_HELPY (2-108): 7.75e-28: 101: 21.43
+	At
		103815 FHIT_ARATH (15-108): 1.02e-26: 99.2: 23.96		FHIT_ARATH (15-108): 1.02e-26: 99.2: 23.96
+	Hs
		890264 FHIT_HUMAN (15-108): 1.1e-24: 93.1: 21.28		FHIT_HUMAN (15-108): 1.1e-24: 93.1: 21.28
+	Zm
		26993 A0A1D6JB03_MAIZE (1-106): 2.29e-22: 88: 24.62		A0A1D6JB03_MAIZE (1-106): 2.29e-22: 88: 24.62