HitPredict

Interactor 1: MYF5 (P13349)
Myogenic factor 5 (Myf-5) (Class C basic helix-loop-helix protein 2) (bHLHc2)
Gene: MYF5

Interactor 2: HEN2 (Q02577)
Helix-loop-helix protein 2 (HEN-2) (Class A basic helix-loop-helix protein 34) (bHLHa34) (Nescient helix loop helix 2) (NSCL-2)
Gene: NHLH2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	HLH~HLH
Common GO terms	GO:0000785 - chromatin GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific GO:0006357 - regulation of transcription by RNA polymerase II and 1 others more... GO:0046983 - protein dimerization activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 255 Hs			1 135
+	Hs
		526739 MYF5_HUMAN (1-255): 8.04e-88: 262: 100.00		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		526750 MYF5_HUMAN (1-255): 8.04e-88: 262: 100.00		SCX_HUMAN (78-133): 2.86e-21: 86.3: 55.36
+	Hs
		545932 MYOD1_HUMAN (7-255): 1.76e-54: 179: 42.67		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		210855 MYOD1_HUMAN (7-255): 1.76e-54: 179: 42.67		TCF21_HUMAN (20-133): 2.08e-24: 94: 25.83
+	Dm
		611973 MYOD_DROME (8-252): 6.38e-55: 181: 37.50		Q9VL05_DROME (69-134): 2.25e-23: 91: 43.94
+	Hs
		551472 MYF6_HUMAN (9-227): 3.4e-45: 153: 38.72		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		546066 MYOG_HUMAN (9-220): 2.93e-44: 150: 38.12		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		551564 ATOH1_HUMAN (62-245): 2.77e-34: 128: 19.25		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		551521 NDF1_HUMAN (4-253): 3.66e-34: 127: 15.73		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		551464 TAL1_HUMAN (62-241): 1.47e-32: 123: 23.20		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Hs
		551577 NDF4_HUMAN (82-251): 2.89e-31: 119: 19.34		TFE2_HUMAN (5-134): 8.2e-26: 102: 22.90
+	Mm
		1141807 NDF1_MOUSE (4-253): 9.24e-34: 126: 15.73		NDF1_MOUSE (68-134): 3.49e-23: 94: 38.81
+	Hs
		568212 TAL1_HUMAN (62-241): 1.47e-32: 123: 23.20		TAL2_HUMAN (78-133): 2.52e-25: 93.9: 58.93
+	Mm
		603895 TAL1_MOUSE (62-241): 5.84e-31: 118: 22.10		TAL1_MOUSE (36-134): 8.21e-25: 98.2: 39.39
+	Mm
		603903 TAL1_MOUSE (62-241): 5.84e-31: 118: 22.10		LYL1_MOUSE (52-134): 3.38e-25: 98.2: 44.58
+	Hs
		568173 TAL1_HUMAN (62-241): 1.47e-32: 123: 23.20		TAL1_HUMAN (52-134): 3.48e-22: 91.3: 44.58
+	Dm
		80872 ATO_DROME (16-139): 4.51e-29: 113: 28.57		DIMM_DROME (13-133): 1.12e-25: 101: 28.35
+	Hs
		524533 TAL1_HUMAN (62-241): 1.47e-32: 123: 23.20		LYL1_HUMAN (52-133): 8.83e-21: 86.7: 46.34
+	Mm
		1142071 HAND2_MOUSE (7-144): 2.82e-29: 111: 22.38		HAND2_MOUSE (76-133): 2.6e-23: 92.1: 50.00
+	Mm
		1142073 HAND2_MOUSE (7-144): 2.82e-29: 111: 22.38		HAND1_MOUSE (76-133): 3.54e-21: 86.3: 51.72