HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ARHG7 (Q14155)
Rho guanine nucleotide exchange factor 7 (Beta-Pix) (COOL-1) (PAK-interacting exchange factor beta) (p85)
Gene: ARHGEF7

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	1.00	0.839

Property	Details
Experiments	31980649, 33961781, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, PH~SH3_1, RhoGEF~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0030032 - lamellipodium assembly GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 803
+	Hs
		209051 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARHG7_HUMAN (1-803): 0: 654: 100.00
+	Hs
		557698 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARHG6_HUMAN (1-802): 1.77e-161: 489: 51.48
+	Hs
		209055 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARHG6_HUMAN (1-802): 1.77e-161: 489: 51.48
+	Hs
		557763 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARHG2_HUMAN (62-801): 1.23e-80: 280: 13.33
+	Hs
		69770 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		B1ALK7_HUMAN (180-797): 8.98e-163: 490: 99.35
+	Hs
		548654 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		548645 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		655500 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		VAV3_HUMAN (1-731): 3.11e-110: 358: 16.73
+	Hs
		411981 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		412098 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		VAV2_HUMAN (1-712): 4.73e-111: 360: 17.04
+	Hs
		548677 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		855781 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		VAV2_HUMAN (1-712): 4.73e-111: 360: 17.04
+	Mm
		1141129 GRB2_MOUSE (1-61): 1.55e-16: 79.4: 29.03		VAV2_MOUSE (1-712): 1.26e-107: 351: 16.38
+	Mm
		654982 GRB2_MOUSE (1-61): 1.55e-16: 79.4: 29.03		VAV_MOUSE (1-754): 3.67e-106: 347: 16.97
+	Hs
		655405 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		FGD2_HUMAN (230-629): 5.88e-78: 266: 18.64
+	Hs
		1078876 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		PREX1_HUMAN (224-582): 1.43e-76: 273: 20.66
+	Hs
		224699 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		KALRN_HUMAN (34-659): 7.34e-70: 253: 13.25
+	Hs
		655386 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ITSN1_HUMAN (21-245): 9.17e-09: 61.3: 14.68
+	Hs
		1078919 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		ITSN2_HUMAN (41-259): 5.37e-11: 68.6: 18.55
+	Hs
		1078828 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		ITSN1_HUMAN (21-245): 9.17e-09: 61.3: 14.68