HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: DLG5 (Q8TDM6)
Disks large homolog 5 (Discs large protein P-dlg) (Placenta and prostate DLG)
Gene: DLG5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Guanylate_kin~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0007165 - signal transduction GO:0030159 - signaling receptor complex adaptor activity
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 377 Hs			1 1919
+	Mm
		223014 NCK2_MOUSE (1-376): 3.75e-90: 277: 66.93		DLG4_MOUSE (1177-1916): 2.2e-156: 500: 20.46
+	Mm
		1113245 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		DLG4_MOUSE (1177-1916): 2.2e-156: 500: 20.46
+	Hs
		655344 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		DLG1_HUMAN (1057-1916): 2.05e-154: 501: 19.62
+	Hs
		129286 P85B_HUMAN (100-376): 1.85e-21: 98.3: 16.61		DLG1_HUMAN (1057-1916): 2.05e-154: 501: 19.62
+	Hs
		412128 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		DLG4_HUMAN (1177-1916): 3.58e-156: 499: 20.46
+	Mm
		403445 SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32		DLG4_MOUSE (1177-1916): 2.2e-156: 500: 20.46
+	Mm
		1141133 GRB2_MOUSE (1-61): 1.55e-16: 79.4: 29.03		DLG4_MOUSE (1177-1916): 2.2e-156: 500: 20.46
+	Hs
		924466 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		DLG4_HUMAN (1177-1916): 3.58e-156: 499: 20.46
+	Rn
		73322 SRC_RAT (5-114): 1.36e-15: 80.1: 22.32		PALS1_RAT (1501-1916): 4.56e-99: 337: 19.50
+	Hs
		522978 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		MPP2_HUMAN (1419-1919): 2.72e-96: 325: 13.41
+	Hs
		1232491 SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76		PALS1_HUMAN (1501-1916): 9.35e-98: 333: 19.50
+	Hs
		209185 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ZO2_HUMAN (1470-1802): 2.6e-11: 71.2: 13.01
+	Dm
		993206 GRAP_DROME (1-61): 1.53e-17: 82.3: 31.15		Q9VHK4_DROME (1359-1806): 8.75e-21: 102: 12.90
+	Hs
		1126376 SH319_HUMAN (8-254): 6.26e-19: 90.5: 19.84		ZO2_HUMAN (1470-1802): 2.6e-11: 71.2: 13.01
+	Hs
		990858 SH319_HUMAN (8-254): 6.26e-19: 90.5: 19.84		ZO1_HUMAN (698-816): 3.52e-09: 64.5: 16.81
+	Hs
		782833 CSK_HUMAN (4-143): 1.92e-12: 70.2: 14.38		ZO2_HUMAN (1470-1802): 2.6e-11: 71.2: 13.01
+	Hs
		782779 CSK_HUMAN (4-143): 1.92e-12: 70.2: 14.38		ZO1_HUMAN (698-816): 3.52e-09: 64.5: 16.81
+	Hs
		1285211 SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76		ZO2_HUMAN (1470-1802): 2.6e-11: 71.2: 13.01