HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: SHAN3 (Q9BYB0)
SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2)
Gene: SHANK3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.57	1.00	0.757

Property	Details
Experiments	21988832, 17474147
Identification Methods	two hybrid, peptide array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH3_2, Ank_2~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1731
+	Hs
		557831 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SHAN2_HUMAN (7-1728): 1.45e-124: 434: 40.04
+	Hs
		298999 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Hs
		655457 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Hs
		412342 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Hs
		1078902 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Hs
		523133 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Hs
		924841 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		SHAN3_HUMAN (1-1731): 0: 596: 100.00
+	Mm
		403435 SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32		SHAN3_MOUSE (1-1731): 8.04e-176: 579: 93.71
+	Mm
		1115913 GRB2_MOUSE (1-61): 1.55e-16: 79.4: 29.03		SHAN3_MOUSE (1-1731): 8.04e-176: 579: 93.71
+	Hs
		655508 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		SHAN2_HUMAN (7-1728): 1.45e-124: 434: 40.04
+	Hs
		523212 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		SHAN2_HUMAN (7-1728): 1.45e-124: 434: 40.04
+	Hs
		924955 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		SHAN2_HUMAN (7-1728): 1.45e-124: 434: 40.04
+	Hs
		557660 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ANK2_HUMAN (117-358): 6.7e-53: 208: 25.27
+	Hs
		557753 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		CSKI2_HUMAN (187-1345): 1.46e-34: 147: 14.44
+	Hs
		209131 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		CSKI2_HUMAN (187-1345): 1.46e-34: 147: 14.44
+	Hs
		655290 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		RIPK4_HUMAN (25-420): 3.28e-78: 280: 16.00
+	Mm
		103593 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		ANK3_MOUSE (120-691): 8.6e-55: 214: 14.98
+	Hs
		924484 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		ANK2_HUMAN (117-358): 6.7e-53: 208: 25.27
+	Hs
		1078859 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		MIB1_HUMAN (129-324): 9.56e-15: 82: 32.50
+	Hs
		655466 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		Q9H4D1_HUMAN (275-465): 9.3e-09: 62.6: 15.39