HitPredict

Interactor 1: RPOA (P20429)
DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) (EC 2.7.7.6) (RNA polymerase subunit alpha) (Transcriptase subunit alpha)
Gene: RPOA

Interactor 2: RPOA (P20429)
DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) (EC 2.7.7.6) (RNA polymerase subunit alpha) (Transcriptase subunit alpha)
Gene: RPOA

Species: Bacillus subtilis subsp. subtilis str. 168

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.53	1.00	0.730

Property	Details
Experiments	21630458, 22512862
Identification Methods	two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	RNA_pol_A_CTD~RNA_pol_A_CTD, RNA_pol_A_CTD~RNA_pol_L, RNA_pol_L~RNA_pol_L
Common GO terms	GO:0000428 - DNA-directed RNA polymerase complex GO:0003677 - DNA binding GO:0003899 - DNA-directed 5'-3' RNA polymerase activity GO:0005737 - cytoplasm GO:0006351 - DNA-templated transcription and 1 others more... GO:0046983 - protein dimerization activity
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 314 Bs			1 314
+	Ec
		462635 RPOA_ECOLI (3-312): 8.99e-107: 315: 45.22		RPOA_ECOLI (3-312): 8.99e-107: 315: 45.22
+	Hs
		583388 RPB3_HUMAN (1-222): 2.6e-62: 200: 16.73		RPB3_HUMAN (1-222): 2.6e-62: 200: 16.73
+	Hs
		169790 RPB3_HUMAN (1-222): 2.6e-62: 200: 16.73		RPAC1_HUMAN (24-232): 1.96e-19: 89: 13.06
+	Sc
		557850 RPB3_YEAST (7-251): 1.02e-33: 127: 13.54		RPB3_YEAST (7-251): 1.02e-33: 127: 13.54
+	Sc
		429613 RPB3_YEAST (7-251): 1.02e-33: 127: 13.54		RPAC1_YEAST (6-226): 1.38e-24: 103: 14.15
+	Sc
		429581 RPAC1_YEAST (6-226): 1.38e-24: 103: 14.15		RPAC1_YEAST (6-226): 1.38e-24: 103: 14.15
+	At
		1107262 NRPB3_ARATH (3-233): 8.07e-26: 106: 14.01		RPD3B_ARATH (3-233): 2.38e-23: 99.4: 12.90