HitPredict

Interactor 1: UBCD1 (P25867)
Ubiquitin-conjugating enzyme E2-17 kDa (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D1) (Protein effete) (Ubiquitin carrier protein) (Ubiquitin-protein ligase)
Gene: eff

Interactor 2: CUL1 (Q24311)
Cullin homolog 1 (Lin-19 homolog protein)
Gene: CUL1

Species: Drosophila melanogaster

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.24	1.00	0.492

Property	Details
Experiments	unassigned4
Identification Methods	experimental interaction detection
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Cullin_Nedd8~UQ_con
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0016567 - protein ubiquitination GO:0031625 - ubiquitin protein ligase binding GO:0045879 - negative regulation of smoothened signaling pathway
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 147 Dm			1 774
+	Hs
		1041136 UB2E3_HUMAN (4-146): 1.83e-73: 219: 65.73		CUL1_HUMAN (3-774): 0: 825: 61.68
+	Hs
		849326 UB2D1_HUMAN (1-147): 1.19e-82: 241: 89.12		CUL4A_HUMAN (17-774): 0: 774: 29.84
+	Hs
		849325 UB2D1_HUMAN (1-147): 1.19e-82: 241: 89.12		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		895791 UB2D3_HUMAN (1-147): 3.31e-81: 237: 93.88		CUL4A_HUMAN (17-774): 0: 774: 29.84
+	Hs
		919724 UB2D2_HUMAN (1-147): 3.06e-82: 239: 94.56		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		1043149 UB2E3_HUMAN (4-146): 1.83e-73: 219: 65.73		CUL4A_HUMAN (17-774): 0: 774: 29.84
+	Hs
		895792 UB2D3_HUMAN (1-147): 3.31e-81: 237: 93.88		CUL4B_HUMAN (3-774): 0: 756: 29.18
+	Hs
		1042304 UB2E2_HUMAN (4-146): 4.09e-74: 221: 65.73		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		1042223 UB2E3_HUMAN (4-146): 1.83e-73: 219: 65.73		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		851812 UB2E1_HUMAN (4-146): 5.46e-72: 215: 65.73		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		896438 UBE2N_HUMAN (4-147): 5.62e-69: 206: 49.31		CUL3_HUMAN (3-774): 0: 771: 27.45
+	Hs
		849324 UB2D1_HUMAN (1-147): 1.19e-82: 241: 89.12		CUL2_HUMAN (7-774): 0: 734: 33.04
+	Hs
		895790 UB2D3_HUMAN (1-147): 3.31e-81: 237: 93.88		CUL2_HUMAN (7-774): 0: 734: 33.04
+	Hs
		828994 UBE2A_HUMAN (2-144): 7.67e-71: 211: 39.16		CUL4B_HUMAN (3-774): 0: 756: 29.18
+	At
		1228143 COP10_ARATH (4-146): 2.98e-58: 180: 58.04		CUL4_ARATH (17-774): 0: 722: 29.41
+	Sp
		804458 UBC4_SCHPO (1-147): 1.62e-80: 235: 87.08		CUL3_SCHPO (12-774): 0: 633: 25.71