HitPredict

Interactor 1: TRI23 (P36406)
E3 ubiquitin-protein ligase TRIM23 (EC 2.3.2.27) (ADP-ribosylation factor domain-containing protein 1) (GTP-binding protein ARD-1) (RING finger protein 46) (RING-type E3 ubiquitin transferase TRIM23) (Tripartite motif-containing protein 23)
Gene: TRIM23

Interactor 2: ARFG1 (Q8N6T3)
ADP-ribosylation factor GTPase-activating protein 1 (ARF GAP 1) (ADP-ribosylation factor 1 GTPase-activating protein) (ARF1 GAP) (ARF1-directed GTPase-activating protein)
Gene: ARFGAP1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	1.00	0.740

Property	Details
Experiments	25416956
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Arf~ArfGap
Common GO terms	GO:0000139 - Golgi membrane GO:0016192 - vesicle-mediated transport
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 574 Hs			1 406
+	Hs
		950523 ARF1_HUMAN (390-569): 5.89e-71: 227: 57.22		ARFG1_HUMAN (1-406): 1.23e-119: 355: 100.00
+	Hs
		912911 ARF6_HUMAN (399-567): 3.05e-87: 269: 54.44		ACAP2_HUMAN (5-387): 3.03e-94: 301: 17.93
+	Hs
		912909 ARF6_HUMAN (399-567): 3.05e-87: 269: 54.44		ACAP1_HUMAN (9-384): 3.07e-62: 215: 12.93
+	Hs
		950563 ARF1_HUMAN (390-569): 5.89e-71: 227: 57.22		ARFG3_HUMAN (1-404): 5.56e-75: 244: 21.13
+	Hs
		574756 ARF4_HUMAN (393-568): 1.47e-70: 226: 55.11		ARFG3_HUMAN (1-404): 5.56e-75: 244: 21.13
+	Hs
		950718 ARF5_HUMAN (392-568): 1.63e-69: 223: 55.37		ARFG3_HUMAN (1-404): 5.56e-75: 244: 21.13
+	Hs
		950522 ARF1_HUMAN (390-569): 5.89e-71: 227: 57.22		ARFG2_HUMAN (3-404): 6.4e-73: 238: 18.88
+	Hs
		574712 ARF4_HUMAN (393-568): 1.47e-70: 226: 55.11		ARFG2_HUMAN (3-404): 6.4e-73: 238: 18.88
+	Hs
		950654 ARF5_HUMAN (392-568): 1.63e-69: 223: 55.37		ARFG2_HUMAN (3-404): 6.4e-73: 238: 18.88
+	At
		285978 ARF2B_ARATH (392-569): 3.28e-72: 230: 57.30		AGD7_ARATH (4-386): 2.81e-65: 217: 24.94
+	Hs
		897406 ARF3_HUMAN (390-569): 5.58e-61: 201: 56.67		ARFG3_HUMAN (1-404): 5.56e-75: 244: 21.13
+	Hs
		574718 ARF4_HUMAN (393-568): 1.47e-70: 226: 55.11		SMAP2_HUMAN (5-356): 1.14e-65: 217: 14.40
+	Hs
		897383 ARF3_HUMAN (390-569): 5.58e-61: 201: 56.67		ARFG2_HUMAN (3-404): 6.4e-73: 238: 18.88
+	Ce
		1353616 Q9U2S4_CAEEL (395-564): 8.87e-85: 263: 51.77		CNT1_CAEEL (8-214): 1.36e-46: 172: 20.91
+	Sc
		503072 ARF1_YEAST (391-568): 4.41e-70: 225: 53.93		GCS1_YEAST (3-332): 3.17e-62: 205: 25.47
+	Hs
		897390 ARF3_HUMAN (390-569): 5.58e-61: 201: 56.67		SMAP2_HUMAN (5-356): 1.14e-65: 217: 14.40
+	Hs
		913181 ARF6_HUMAN (399-567): 3.05e-87: 269: 54.44		ASAP1_HUMAN (6-89): 2.45e-12: 70.9: 38.10
+	Sc
		503086 ARF1_YEAST (391-568): 4.41e-70: 225: 53.93		GLO3_YEAST (6-87): 6.22e-17: 84.3: 46.34
+	Sc
		580854 ARF2_YEAST (391-567): 2.59e-70: 225: 53.11		GLO3_YEAST (6-87): 6.22e-17: 84.3: 46.34
+	Hs
		950577 ARF1_HUMAN (390-569): 5.89e-71: 227: 57.22		ASAP1_HUMAN (6-89): 2.45e-12: 70.9: 38.10