HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: BUL2 (Q03758)
Ubiquitin ligase-binding protein BUL2
Gene: BUL2

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.78	0.60	0.687

Property	Details
Experiments	9931424, 29129641, 20489023, 22307975
Identification Methods	two hybrid, affinity chromatography technology, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000151 - ubiquitin ligase complex GO:0005737 - cytoplasm GO:0043162 - ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:1990306 - RSP5-BUL ubiquitin ligase complex GO:2000397 - positive regulation of ubiquitin-dependent endocytosis
Homologous interactions	3

Homologous interactions

Color coding for hit scores

	1 809 Sc			1 920
+	Sc
		765317 RSP5_YEAST (1-809): 0: 825: 100.00		BUL1_YEAST (82-918): 0: 1230: 54.44
+	Sc
		765344 RSP5_YEAST (1-809): 0: 825: 100.00		BSC5_YEAST (77-525): 0: 585: 35.15
+	Sc
		765343 RSP5_YEAST (1-809): 0: 825: 100.00		YN97_YEAST (643-916): 1.14e-119: 366: 30.00