HitPredict

Interactor 1: ZAP70 (P43404)
Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase)
Gene: ZAP70

Interactor 2: GRB2 (Q60631)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.33	1.00	0.575

Property	Details
Experiments	24584089
Identification Methods	affinity chromatography technology
Interaction Type	association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1, SH2~SH2, SH2~SH3_1
Common GO terms	GO:0001784 - phosphotyrosine residue binding GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0005911 - cell-cell junction and 1 others more... GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 618 Mm			1 217
+	Hs
		796465 ZAP70_HUMAN (1-618): 0: 615: 93.39		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Mm
		411920 ZAP70_MOUSE (1-618): 0: 635: 100.00		LCK_MOUSE (3-202): 5.14e-38: 139: 28.44
+	Hs
		796538 KSYK_HUMAN (1-598): 0: 543: 54.05		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Hs
		411632 ZAP70_HUMAN (1-618): 0: 615: 93.39		LCK_HUMAN (3-202): 7.66e-38: 138: 28.91
+	Hs
		782752 ZAP70_HUMAN (1-618): 0: 615: 93.39		CSK_HUMAN (2-139): 4.25e-24: 100: 36.24
+	Mm
		654974 ZAP70_MOUSE (1-618): 0: 635: 100.00		VAV_MOUSE (3-60): 1.12e-15: 76.9: 43.10
+	Hs
		337385 ZAP70_HUMAN (1-618): 0: 615: 93.39		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		548655 ZAP70_HUMAN (1-618): 0: 615: 93.39		VAV_HUMAN (3-60): 1.14e-15: 76.9: 43.10
+	Hs
		412075 ZAP70_HUMAN (1-618): 0: 615: 93.39		FYN_HUMAN (161-215): 4.88e-13: 69.2: 31.58
+	Hs
		411633 KSYK_HUMAN (1-598): 0: 543: 54.05		LCK_HUMAN (3-202): 7.66e-38: 138: 28.91
+	Gg
		94447 KSYK_CHICK (1-601): 0: 568: 53.12		CSK_CHICK (2-139): 5.18e-24: 100: 35.57
+	Hs
		557633 KSYK_HUMAN (1-598): 0: 543: 54.05		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		548656 KSYK_HUMAN (1-598): 0: 543: 54.05		VAV_HUMAN (3-60): 1.14e-15: 76.9: 43.10
+	Hs
		412076 KSYK_HUMAN (1-598): 0: 543: 54.05		FYN_HUMAN (161-215): 4.88e-13: 69.2: 31.58
+	Hs
		454806 KSYK_HUMAN (1-598): 0: 543: 54.05		FGR_HUMAN (161-215): 5.31e-12: 66.1: 33.33
+	Hs
		796531 KSYK_HUMAN (1-598): 0: 543: 54.05		VAV2_HUMAN (3-56): 6.02e-11: 63.1: 38.18
+	Hs
		275178 KSYK_HUMAN (1-598): 0: 543: 54.05		GRAP2_HUMAN (3-55): 7.92e-11: 62.5: 39.62
+	Hs
		522845 KSYK_HUMAN (1-598): 0: 543: 54.05		SRC_HUMAN (160-215): 1.74e-10: 61.9: 31.03
+	Hs
		433635 KSYK_HUMAN (1-598): 0: 543: 54.05		LYN_HUMAN (136-215): 4.12e-10: 60.7: 24.39
+	Hs
		433772 EPHA7_HUMAN (55-612): 7.2e-157: 477: 23.16		LYN_HUMAN (136-215): 4.12e-10: 60.7: 24.39