HitPredict

Interactor 1: NASP (P49321)
Nuclear autoantigenic sperm protein (NASP)
Gene: NASP

Interactor 2: H33 (P84243)
Histone H3.3
Gene: H3-3A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.973

Property	Details
Experiments	28514442, 33961781, 31839598, 25416956, 36089195, 36949045, 36652389, 14718166, 20504901
Identification Methods	affinity chromatography technology, cross-linking study, two hybrid, proximity labelling technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Histone~SHNi-TPR
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0006334 - nucleosome assembly GO:0008584 - male gonad development GO:0032991 - protein-containing complex
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 788 Hs			1 136
+	Hs
		881034 NASP_HUMAN (1-788): 1.95e-140: 435: 100.00		H31_HUMAN (1-136): 3.09e-60: 183: 96.32
+	Hs
		881057 NASP_HUMAN (1-788): 1.95e-140: 435: 100.00		H31T_HUMAN (1-136): 2.59e-59: 181: 93.38
+	Hs
		881065 NASP_HUMAN (1-788): 1.95e-140: 435: 100.00		H32_HUMAN (1-136): 5.34e-59: 180: 97.06
+	Hs
		881059 NASP_HUMAN (1-788): 1.95e-140: 435: 100.00		H37_HUMAN (1-136): 4.56e-57: 175: 94.12
+	Hs
		881060 NASP_HUMAN (1-788): 1.95e-140: 435: 100.00		H3C_HUMAN (1-136): 2e-56: 173: 95.59
+	Mm
		73041 B1AU75_MOUSE (1-788): 4.16e-117: 373: 79.62		H31_MOUSE (1-136): 3.09e-60: 183: 96.32
+	Mm
		73043 B1AU75_MOUSE (1-788): 4.16e-117: 373: 79.62		H33_MOUSE (1-136): 1.13e-59: 182: 100.00
+	Mm
		73042 B1AU75_MOUSE (1-788): 4.16e-117: 373: 79.62		H32_MOUSE (1-136): 5.34e-59: 180: 97.06
+	Hs
		998438 NIPBL_HUMAN (80-756): 2.34e-100: 343: 12.41		H31_HUMAN (1-136): 3.09e-60: 183: 96.32
+	Hs
		1016949 NIPBL_HUMAN (80-756): 2.34e-100: 343: 12.41		H33_HUMAN (1-136): 1.13e-59: 182: 100.00
+	Hs
		883582 NIPBL_HUMAN (80-756): 2.34e-100: 343: 12.41		CENPA_HUMAN (9-133): 2.43e-45: 146: 50.76
+	Hs
		998611 TITIN_HUMAN (205-754): 1.25e-13: 77.7: 9.70		H31_HUMAN (1-136): 3.09e-60: 183: 96.32
+	Hs
		1258894 TITIN_HUMAN (205-754): 1.25e-13: 77.7: 9.70		H32_HUMAN (1-136): 5.34e-59: 180: 97.06
+	Hs
		998464 WDR87_HUMAN (115-735): 6.08e-10: 65.4: 16.16		H31_HUMAN (1-136): 3.09e-60: 183: 96.32
+	Sp
		489140 SIM3_SCHPO (426-669): 1.95e-10: 65.4: 21.46		H31_SCHPO (1-136): 1.12e-57: 177: 91.91
+	Sp
		1462866 SIM3_SCHPO (426-669): 1.95e-10: 65.4: 21.46		CENPA_SCHPO (26-133): 9.59e-42: 136: 63.16