HitPredict

Interactor 1: ACTB (P60709)
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene: ACTB

Interactor 2: DEST (P60981)
Destrin (Actin-depolymerizing factor) (ADF)
Gene: DSTN

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.974

Property	Details
Experiments	25416956, 16189514, 29892012, 32296183, 35271311, 37071682, 31515488, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, cross-linking study, two hybrid array, validated two hybrid, anti tag coimmunoprecipitation, two hybrid prey pooling approach, two hybrid pooling approach
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005737 - cytoplasm GO:0015629 - actin cytoskeleton GO:0048870 - cell motility GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Hs			1 165
+	Hs
		618104 ACTBL_HUMAN (2-375): 0: 735: 91.71		COF1_HUMAN (1-165): 5.13e-70: 210: 71.08
+	Hs
		1122425 ACTBL_HUMAN (2-375): 0: 735: 91.71		TWF2_HUMAN (3-152): 3.92e-31: 116: 18.30
+	Hs
		894665 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (1-165): 6.05e-74: 220: 100.00
+	Hs
		894652 ACTG_HUMAN (1-375): 0: 601: 98.93		DEST_HUMAN (1-165): 6.05e-74: 220: 100.00
+	Mm
		578583 ACTB_MOUSE (1-375): 0: 603: 100.00		COF1_MOUSE (1-165): 2.57e-70: 211: 71.08
+	Hs
		1092828 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (1-165): 4.17e-70: 210: 68.68
+	Hs
		618038 ACTB_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (1-165): 5.13e-70: 210: 71.08
+	Hs
		618096 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (1-165): 5.13e-70: 210: 71.08
+	Rn
		799735 ACTB_RAT (1-375): 0: 603: 100.00		COF1_RAT (1-165): 4.8e-70: 210: 71.08
+	Hs
		891817 ACTB_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (1-165): 4.17e-70: 210: 68.68
+	Hs
		892124 ACTB_HUMAN (1-375): 0: 603: 100.00		COF2_HUMAN (1-165): 4.17e-70: 210: 68.68
+	Hs
		618049 ACTG_HUMAN (1-375): 0: 601: 98.93		COF1_HUMAN (1-165): 5.13e-70: 210: 71.08
+	Hs
		929891 ACTG_HUMAN (1-375): 0: 601: 98.93		Q549N0_HUMAN (1-165): 4.17e-70: 210: 68.68
+	Hs
		930059 ACTG_HUMAN (1-375): 0: 601: 98.93		COF2_HUMAN (1-165): 4.17e-70: 210: 68.68
+	Hs
		891728 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF1_HUMAN (3-152): 1.54e-37: 133: 20.26
+	Hs
		929835 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF1_HUMAN (3-152): 1.54e-37: 133: 20.26
+	Hs
		891650 ACTB_HUMAN (1-375): 0: 603: 100.00		GMFB_HUMAN (11-153): 1.73e-34: 119: 21.92
+	Hs
		894697 ACTG_HUMAN (1-375): 0: 601: 98.93		GMFB_HUMAN (11-153): 1.73e-34: 119: 21.92
+	Hs
		891834 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF2_HUMAN (3-152): 3.92e-31: 116: 18.30
+	Hs
		929897 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF2_HUMAN (3-152): 3.92e-31: 116: 18.30