HitPredict

Interactor 1: ACTB (P60709)
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene: ACTB

Interactor 2: TWF2 (Q6IBS0)
Twinfilin-2 (A6-related protein) (hA6RP) (Protein tyrosine kinase 9-like) (Twinfilin-1-like protein)
Gene: TWF2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	28514442, 33961781, 35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005524 - ATP binding GO:0005737 - cytoplasm GO:0005884 - actin filament GO:0030027 - lamellipodium GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Hs			1 349
+	Hs
		1122425 ACTBL_HUMAN (2-375): 0: 735: 91.71		TWF2_HUMAN (1-349): 1.13e-170: 480: 100.00
+	Hs
		929897 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF2_HUMAN (1-349): 1.13e-170: 480: 100.00
+	Hs
		891728 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF1_HUMAN (1-344): 4.14e-154: 438: 65.41
+	Hs
		929835 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF1_HUMAN (1-344): 4.14e-154: 438: 65.41
+	Hs
		618104 ACTBL_HUMAN (2-375): 0: 735: 91.71		COF1_HUMAN (3-138): 3.06e-32: 120: 17.95
+	Hs
		891649 ACTB_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (3-138): 2.92e-40: 141: 21.29
+	Hs
		891650 ACTB_HUMAN (1-375): 0: 603: 100.00		GMFB_HUMAN (178-312): 1.08e-40: 141: 22.14
+	Hs
		894665 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (3-138): 2.92e-40: 141: 21.29
+	Hs
		894652 ACTG_HUMAN (1-375): 0: 601: 98.93		DEST_HUMAN (3-138): 2.92e-40: 141: 21.29
+	Hs
		894697 ACTG_HUMAN (1-375): 0: 601: 98.93		GMFB_HUMAN (178-312): 1.08e-40: 141: 22.14
+	Hs
		1092828 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (3-138): 6.86e-35: 127: 21.80
+	Hs
		891817 ACTB_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (3-138): 6.86e-35: 127: 21.80
+	Hs
		892124 ACTB_HUMAN (1-375): 0: 603: 100.00		COF2_HUMAN (3-138): 6.86e-35: 127: 21.80
+	Hs
		929891 ACTG_HUMAN (1-375): 0: 601: 98.93		Q549N0_HUMAN (3-138): 6.86e-35: 127: 21.80
+	Hs
		930059 ACTG_HUMAN (1-375): 0: 601: 98.93		COF2_HUMAN (3-138): 6.86e-35: 127: 21.80
+	Mm
		578583 ACTB_MOUSE (1-375): 0: 603: 100.00		COF1_MOUSE (3-138): 6.36e-33: 122: 18.59
+	Hs
		618038 ACTB_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (3-138): 3.06e-32: 120: 17.95
+	Hs
		618096 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (3-138): 3.06e-32: 120: 17.95
+	Rn
		799735 ACTB_RAT (1-375): 0: 603: 100.00		COF1_RAT (3-138): 1.73e-32: 120: 17.95
+	Hs
		618049 ACTG_HUMAN (1-375): 0: 601: 98.93		COF1_HUMAN (3-138): 3.06e-32: 120: 17.95