HitPredict

Interactor 1: ACTB (P60709)
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene: ACTB

Interactor 2: COF2 (Q9Y281)
Cofilin-2 (Cofilin, muscle isoform)
Gene: CFL2

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.975

Property	Details
Experiments	21516116, 25416956, 16189514, 25910212, 32296183, 35271311, 31515488, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, pull down, two hybrid array, two hybrid pooling approach, two hybrid bait and prey pooling approach, validated two hybrid, two hybrid prey pooling approach, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005615 - extracellular space GO:0005737 - cytoplasm GO:0015629 - actin cytoskeleton GO:0016363 - nuclear matrix GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Hs			1 166
+	Hs
		618104 ACTBL_HUMAN (2-375): 0: 735: 91.71		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		1122425 ACTBL_HUMAN (2-375): 0: 735: 91.71		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42
+	Hs
		1092828 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Mm
		578583 ACTB_MOUSE (1-375): 0: 603: 100.00		COF1_MOUSE (1-166): 1.72e-73: 219: 80.72
+	Hs
		891817 ACTB_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		618038 ACTB_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		618096 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Rn
		799735 ACTB_RAT (1-375): 0: 603: 100.00		COF1_RAT (1-166): 3.36e-73: 218: 81.33
+	Hs
		929891 ACTG_HUMAN (1-375): 0: 601: 98.93		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		930059 ACTG_HUMAN (1-375): 0: 601: 98.93		COF2_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		618049 ACTG_HUMAN (1-375): 0: 601: 98.93		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		891649 ACTB_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		894665 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		894652 ACTG_HUMAN (1-375): 0: 601: 98.93		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		891728 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF1_HUMAN (3-156): 3.59e-33: 121: 21.66
+	Hs
		929835 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF1_HUMAN (3-156): 3.59e-33: 121: 21.66
+	Hs
		891650 ACTB_HUMAN (1-375): 0: 603: 100.00		GMFB_HUMAN (1-154): 2.3e-33: 116: 19.11
+	Hs
		894697 ACTG_HUMAN (1-375): 0: 601: 98.93		GMFB_HUMAN (1-154): 2.3e-33: 116: 19.11
+	Hs
		891834 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42
+	Hs
		929897 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42