HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: RN185 (Q96GF1)
E3 ubiquitin-protein ligase RNF185 (EC 2.3.2.27) (RING finger protein 185)
Gene: RNF185

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.78	1.00	0.885

Property	Details
Experiments	32296183, 19549727, 17558393, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4
Common GO terms	GO:0006511 - ubiquitin-dependent protein catabolic process GO:0060340 - positive regulation of type I interferon-mediated signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 200 Hs			1 192
+	Xl
		1140146 Q8AVM8_XENLA (1-200): 1.98e-82: 244: 97.00		Q5XGX4_XENLA (1-192): 1.06e-55: 176: 91.15
+	Hs
		1292368 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN185_HUMAN (1-192): 2.35e-56: 178: 100.00
+	Hs
		849409 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RN185_HUMAN (1-192): 2.35e-56: 178: 100.00
+	Hs
		896364 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38
+	Hs
		1307272 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38
+	Hs
		849424 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38
+	Hs
		896381 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI39_HUMAN (12-144): 3.88e-26: 106: 18.80
+	Hs
		896395 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		AMFR_HUMAN (27-171): 2.12e-24: 101: 19.18
+	Hs
		849314 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI26_HUMAN (26-132): 1.42e-27: 109: 23.48
+	Hs
		849406 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI11_HUMAN (27-130): 9.09e-27: 107: 25.00
+	Hs
		1336390 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI39_HUMAN (12-144): 3.88e-26: 106: 18.80
+	Hs
		584907 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RO52_HUMAN (26-144): 2e-26: 106: 20.17
+	Hs
		849366 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI22_HUMAN (32-155): 2.91e-26: 106: 18.75
+	Hs
		849453 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI39_HUMAN (12-144): 3.88e-26: 106: 18.80
+	Hs
		584923 UB2D2_HUMAN (4-154): 7.76e-75: 223: 41.06		RO52_HUMAN (26-144): 2e-26: 106: 20.17
+	Hs
		1356614 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		AMFR_HUMAN (27-171): 2.12e-24: 101: 19.18
+	Hs
		849484 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		AMFR_HUMAN (27-171): 2.12e-24: 101: 19.18
+	Hs
		749302 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		BRCA1_HUMAN (38-88): 2.19e-11: 64: 35.29
+	Hs
		749252 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		BRCA1_HUMAN (38-88): 2.19e-11: 64: 35.29
+	Hs
		749339 UB2D2_HUMAN (4-154): 7.76e-75: 223: 41.06		BRCA1_HUMAN (38-88): 2.19e-11: 64: 35.29