HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: PKN1 (Q16512)
Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
Gene: PKN1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.929

Property	Details
Experiments	12773565, 9446575, 31871319, 9535835, 10619026
Identification Methods	pull down, two hybrid, affinity chromatography technology, far western blotting, x-ray crystallography
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	HR1~Ras, Pkinase~Ras
Common GO terms	GO:0005634 - nucleus GO:0005768 - endosome GO:0005829 - cytosol GO:0006357 - regulation of transcription by RNA polymerase II GO:0030496 - midbody and 1 others more... GO:0032154 - cleavage furrow
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 942
+	Hs
		925307 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN1_HUMAN (1-942): 0: 1097: 97.90
+	Hs
		900514 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		925308 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		436326 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		951021 RHOG_HUMAN (1-193): 2.83e-77: 231: 54.40		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		894056 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		900594 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN3_HUMAN (36-939): 0: 867: 49.84
+	Ce
		107919 RAC1_CAEEL (1-193): 2.07e-80: 238: 55.44		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Ce
		104463 G5EBV3_CAEEL (2-193): 1.08e-77: 232: 50.52		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Hs
		681775 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		AKT1_HUMAN (587-941): 7.64e-153: 461: 43.06
+	Sp
		736859 RHO1_SCHPO (1-193): 2.83e-94: 274: 66.83		PCK2_SCHPO (587-937): 1.08e-110: 367: 48.88
+	Sp
		736865 RHO5_SCHPO (4-193): 1.09e-93: 273: 65.31		PCK2_SCHPO (587-937): 1.08e-110: 367: 48.88
+	Dm
		52723 RAC1_DROME (1-193): 2.11e-77: 231: 54.12		PKN_DROME (590-942): 1.28e-122: 403: 66.94
+	Dm
		52739 RAC1_DROME (1-193): 2.11e-77: 231: 54.12		A1Z7T1_DROME (590-942): 2e-119: 398: 66.94
+	Sc
		419673 RHO1_YEAST (3-188): 1.51e-97: 283: 73.12		KPC1_YEAST (547-938): 1.45e-100: 342: 43.04
+	Sp
		736851 RHO1_SCHPO (1-193): 2.83e-94: 274: 66.83		PCK1_SCHPO (557-939): 2.67e-102: 343: 47.40
+	Sp
		736856 RHO5_SCHPO (4-193): 1.09e-93: 273: 65.31		PCK1_SCHPO (557-939): 2.67e-102: 343: 47.40
+	Hs
		900406 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		KPCD_HUMAN (608-938): 7.48e-103: 337: 50.30
+	Hs
		436269 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		KPCD_HUMAN (608-938): 7.48e-103: 337: 50.30
+	Rn
		448411 RAC1_RAT (1-193): 8.86e-80: 237: 57.22		KPCD_RAT (612-938): 3.16e-101: 332: 49.39