HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: NGEF (Q8N5V2)
Ephexin-1 (Eph-interacting exchange protein) (Neuronal guanine nucleotide exchange factor)
Gene: NGEF

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

Property	Details
Experiments	32203420, 31871319, 18256687
Identification Methods	fluorescent resonance energy transfer, affinity chromatography technology, pull down
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PH~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 710
+	Hs
		900786 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGQ_HUMAN (20-691): 1.64e-119: 380: 27.26
+	Hs
		900416 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHG5_HUMAN (3-691): 4.07e-113: 375: 34.20
+	Hs
		900663 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGJ_HUMAN (10-691): 1.19e-115: 368: 30.24
+	Hs
		900947 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHG4_HUMAN (9-644): 1.24e-101: 328: 15.36
+	Hs
		548672 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV_HUMAN (152-709): 1.25e-98: 324: 15.87
+	Hs
		167078 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGB_HUMAN (3-708): 5.04e-93: 318: 15.16
+	Mm
		650205 RHOA_MOUSE (1-193): 4.1e-81: 240: 99.48		RGRF1_MOUSE (76-638): 4.34e-90: 308: 15.78
+	Hs
		901037 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV3_HUMAN (167-708): 6.57e-93: 309: 12.16
+	Hs
		302670 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGF_HUMAN (48-692): 2.22e-90: 302: 29.40
+	Hs
		900616 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PREX2_HUMAN (251-614): 2.77e-86: 299: 19.48
+	Hs
		900916 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ECT2_HUMAN (146-681): 1.79e-88: 298: 15.87
+	Hs
		855779 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV2_HUMAN (167-682): 4.63e-88: 296: 13.88
+	Hs
		900985 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGC_HUMAN (22-647): 4.74e-84: 292: 14.82
+	Hs
		900718 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PREX1_HUMAN (223-613): 3.34e-83: 290: 19.66
+	Hs
		156395 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RGRF2_HUMAN (76-598): 4.72e-83: 288: 15.48
+	Hs
		156397 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		RGRF2_HUMAN (76-598): 4.72e-83: 288: 15.48
+	Hs
		900813 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGH_HUMAN (5-568): 4.88e-81: 284: 17.07
+	Hs
		297123 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKHG5_HUMAN (165-596): 2.1e-68: 244: 20.59
+	Hs
		279851 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		TRIO_HUMAN (262-658): 2.58e-58: 216: 19.19
+	Sp
		1000083 RHO1_SCHPO (1-193): 2.83e-94: 274: 66.83		RGF3_SCHPO (271-530): 5.67e-11: 68.2: 25.46