HitPredict

Interactor 1: SUMO2 (P61956)
Small ubiquitin-related modifier 2 (SUMO-2) (HSMT3) (SMT3 homolog 2) (SUMO-3) (Sentrin-2) (Ubiquitin-like protein SMT3B) (Smt3B)
Gene: SUMO2

Interactor 2: PIAS4 (Q8N2W9)
E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma)
Gene: PIAS4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.85	1.00	0.924

Property	Details
Experiments	22578841, 19394292, 32786267, 15383276, 16524884
Identification Methods	pull down, two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Rad60-SLD~zf-MIZ
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0016605 - PML body GO:0016925 - protein sumoylation GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 95 Hs			1 510
+	Hs
		879136 SUMO3_HUMAN (1-94): 3.76e-41: 132: 96.81		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		928135 SUMO1_HUMAN (1-93): 7.76e-35: 116: 45.36		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Rn
		78200 SUMO3_RAT (1-94): 2.04e-42: 135: 95.75		B5DFF9_RAT (1-510): 0: 628: 87.96
+	Rn
		78199 SUMO1_RAT (1-93): 7.76e-35: 116: 45.36		B5DFF9_RAT (1-510): 0: 628: 87.96
+	Hs
		902827 SUMO2_HUMAN (1-95): 3.56e-44: 139: 100.00		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		879167 SUMO3_HUMAN (1-94): 3.76e-41: 132: 96.81		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278020 SUMO2_HUMAN (1-95): 3.56e-44: 139: 100.00		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		278253 SUMO2_HUMAN (1-95): 3.56e-44: 139: 100.00		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Rn
		1140374 SUMO3_RAT (1-94): 2.04e-42: 135: 95.75		PIAS2_RAT (3-504): 0: 522: 44.74
+	Mm
		222291 SUMO1_MOUSE (1-93): 7.76e-35: 116: 45.36		PIAS3_MOUSE (3-509): 0: 541: 46.20
+	Hs
		928241 SUMO1_HUMAN (1-93): 7.76e-35: 116: 45.36		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278009 SUMO3_HUMAN (1-94): 3.76e-41: 132: 96.81		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		278250 SUMO3_HUMAN (1-94): 3.76e-41: 132: 96.81		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Hs
		278040 SUMO1_HUMAN (1-93): 7.76e-35: 116: 45.36		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		278256 SUMO1_HUMAN (1-93): 7.76e-35: 116: 45.36		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Dm
		332592 O97102_DROME (1-94): 1.24e-37: 123: 68.09		Q9XYM5_DROME (11-461): 2.02e-143: 424: 40.08
+	At
		1147334 SUMO2_ARATH (3-93): 5.57e-38: 124: 51.65		SIZ1_ARATH (8-415): 4.87e-80: 269: 20.65
+	At
		1147333 SUMO3_ARATH (1-94): 1.6e-36: 120: 40.43		SIZ1_ARATH (8-415): 4.87e-80: 269: 20.65
+	At
		878961 SUMO1_ARATH (1-91): 8.61e-36: 118: 47.25		SIZ1_ARATH (8-415): 4.87e-80: 269: 20.65
+	Rn
		89025 SUMO3_RAT (1-94): 2.04e-42: 135: 95.75		D4AE97_RAT (73-419): 3.81e-71: 247: 23.42