HitPredict

Interactor 1: RBX1 (P62877)
E3 ubiquitin-protein ligase RBX1 (EC 2.3.2.27) (EC 2.3.2.32) (E3 ubiquitin-protein transferase RBX1) (Protein ZYP) (RING finger protein 75) (RING-box protein 1) (Rbx1) (Regulator of cullins 1) (ROC1) [Cleaved into: E3 ubiquitin-protein ligase RBX1, N-terminally processed (E3 ubiquitin-protein transferase RBX1, N-terminally processed)]
Gene: RBX1

Interactor 2: CUL4B (Q13620)
Cullin-4B (CUL-4B)
Gene: CUL4B

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.97 1.00 0.986

The method score is based on:

Property Details
Experiments 26496610, 27113764, 28446751, 29499938, 30945288, 32140073, 31973889, 33969670, 22038470, 19250909, 30349055, 12481031, 33961781, 33504946, 34026424, 12609982, 23238014
Identification Methods affinity chromatography technology, two hybrid, pull down, enzymatic study, anti tag coimmunoprecipitation
Interaction Type physical association, direct interaction, association

The annotation score is based on:

Property Details
Interacting Pfam domains Cullin~zf-rbx1, Cullin_Nedd8~zf-rbx1
Common GO terms GO:0000209 - protein polyubiquitination
GO:0005634 - nucleus
GO:0005654 - nucleoplasm
GO:0005829 - cytosol
GO:0006511 - ubiquitin-dependent protein catabolic process
and 7 others

more...
Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 108
Hs

1 913
+ Mm
+ Mm
+ Hs
+ Dm
+ At
+ At
+ At
+ Hs
+ Dm
+ Hs
+ Mm
+ At
+ Hs
+ Dm
+ Sp
+ Dm
+ Ce
+ Sp
+ Hs
+ Ce

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Last updated: 15 Jul 2024