HitPredict

Interactor 1: RS27A (P62983)
Ubiquitin-ribosomal protein eS31 fusion protein (Ubiquitin carboxyl extension protein 80) [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S27a)]
Gene: Rps27a

Interactor 2: HUWE1 (Q7TMY8)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (E3Histone) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	28671696
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HECT~ubiquitin, UBA~ubiquitin, UBM~ubiquitin
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 156 Mm			1 4377
+	Mm
		476858 UBC_MOUSE (1-119): 1.1e-36: 134: 67.48		HUWE1_MOUSE (1-4377): 0: 3281: 100.00
+	Hs
		924336 RL40_HUMAN (1-125): 3e-42: 138: 64.80		HUWE1_HUMAN (1-4377): 0: 3263: 96.62
+	Hs
		125347 RS27A_HUMAN (1-156): 5.74e-74: 219: 99.36		WWP2_HUMAN (3581-4375): 0: 631: 26.00
+	Hs
		924156 RS27A_HUMAN (1-156): 5.74e-74: 219: 99.36		WWP1_HUMAN (3644-4375): 0: 630: 25.00
+	Rn
		1140970 UBC_RAT (1-119): 1.47e-36: 134: 68.29		Q5YB86_RAT (3543-4375): 0: 689: 24.50
+	Sc
		408371 RS31_YEAST (1-151): 4.08e-59: 182: 82.12		RSP5_YEAST (3618-4375): 0: 589: 26.45
+	Hs
		924414 RL40_HUMAN (1-125): 3e-42: 138: 64.80		SMUF2_HUMAN (3803-4374): 0: 631: 35.19
+	Sp
		145416 RS27A_SCHPO (1-151): 5.65e-56: 174: 80.80		PUB3_SCHPO (3698-4375): 0: 586: 28.59
+	Sp
		145417 RS27B_SCHPO (1-151): 4.44e-56: 174: 80.80		PUB3_SCHPO (3698-4375): 0: 586: 28.59
+	Hs
		125112 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		WWP2_HUMAN (3581-4375): 0: 631: 26.00
+	Sc
		408448 RS31_YEAST (1-151): 4.08e-59: 182: 82.12		TOM1_YEAST (3958-4377): 5.67e-157: 553: 51.78
+	Hs
		474953 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		NEDD4_HUMAN (3124-4375): 0: 605: 21.85
+	Sp
		145418 RL402_SCHPO (1-125): 3.2e-39: 130: 63.20		PUB3_SCHPO (3698-4375): 0: 586: 28.59
+	At
		1091762 UBQ3_ARATH (1-77): 2.1e-35: 126: 94.81		UPL2_ARATH (2954-4377): 5.76e-163: 573: 22.73
+	Dm
		89880 RL40_DROME (1-125): 5.96e-42: 137: 64.80		D5SHR3_DROME (3968-4376): 7.42e-178: 556: 37.38
+	Sc
		477647 RL40A_YEAST (1-125): 1.03e-40: 134: 64.00		TOM1_YEAST (3958-4377): 5.67e-157: 553: 51.78
+	Dm
		1376481 Q9W418_DROME (1-77): 1.59e-34: 127: 100.00		Q9W325_DROME (3968-4376): 7.98e-178: 556: 37.38
+	Hs
		475078 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		UBE3B_HUMAN (3774-4377): 2.2e-169: 556: 25.71
+	Mm
		477022 UBC_MOUSE (1-119): 1.1e-36: 134: 67.48		UBE3B_MOUSE (3773-4377): 2.91e-165: 544: 25.30
+	At
		1091758 UBQ3_ARATH (1-77): 2.1e-35: 126: 94.81		UPL1_ARATH (1277-2947): 5.86e-117: 422: 13.93