HitPredict

Interactor 1: UBC9 (P63279)
SUMO-conjugating enzyme UBC9 (EC 2.3.2.-) (RING-type E3 SUMO transferase UBC9) (SUMO-protein ligase) (Ubiquitin carrier protein 9) (Ubiquitin carrier protein I) (Ubiquitin-conjugating enzyme E2 I) (Ubiquitin-protein ligase I) (p18)
Gene: UBE2I

Interactor 2: SAE1 (Q9UBE0)
SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed]
Gene: SAE1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.980

Property	Details
Experiments	26496610, 28514442, 17353931, 15229330, 33961781, 23601649, 19250909, 35271311, 25412743, 12641448
Identification Methods	affinity chromatography technology, pull down, enzymatic study, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	ThiF~UQ_con
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0016925 - protein sumoylation GO:0044388 - small protein activating enzyme binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 158 Hs			1 346
+	At
		1114935 SCE1_ARATH (2-155): 5.4e-72: 215: 66.23		SAE1A_ARATH (6-344): 1.7e-98: 296: 30.84
+	Dm
		1181392 Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44		Q7KSM5_DROME (2-346): 5.44e-93: 282: 39.42
+	Hs
		919796 UB2D2_HUMAN (4-157): 2.44e-74: 220: 35.07		UBA3_HUMAN (28-337): 4.67e-82: 258: 17.68
+	Hs
		48104 UB2D1_HUMAN (4-157): 7.96e-75: 221: 33.12		UBA6_HUMAN (17-295): 1.69e-45: 168: 14.48
+	Hs
		48107 UB2D2_HUMAN (4-157): 2.44e-74: 220: 35.07		UBA6_HUMAN (17-295): 1.69e-45: 168: 14.48
+	Hs
		48106 UB2D3_HUMAN (4-157): 6.67e-73: 216: 35.07		UBA6_HUMAN (17-295): 1.69e-45: 168: 14.48
+	At
		1115004 SCE1_ARATH (2-155): 5.4e-72: 215: 66.23		SAE2_ARATH (29-183): 5.38e-43: 159: 24.05
+	Hs
		607190 UB2D4_HUMAN (4-157): 3.74e-75: 222: 34.42		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607026 UB2D1_HUMAN (4-157): 7.96e-75: 221: 33.12		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607050 UB2D2_HUMAN (4-157): 2.44e-74: 220: 35.07		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Sc
		549788 UBC4_YEAST (1-156): 1.22e-72: 216: 35.90		UBA1_YEAST (16-232): 6.94e-40: 151: 21.61
+	Hs
		607039 UB2D3_HUMAN (4-157): 6.67e-73: 216: 35.07		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Sp
		293848 UBC4_SCHPO (4-156): 2.83e-72: 215: 36.60		UBA1_SCHPO (18-236): 6.77e-39: 148: 17.80
+	Hs
		607055 UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607136 UB2E2_HUMAN (3-156): 7.29e-69: 208: 33.77		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607129 UB2E3_HUMAN (3-156): 2.14e-68: 207: 33.77		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607028 UB2E1_HUMAN (3-156): 7.23e-67: 203: 33.12		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607023 UBE2A_HUMAN (1-154): 3.37e-67: 202: 41.56		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		607054 UBE2B_HUMAN (1-154): 1.37e-66: 201: 41.56		UBA1_HUMAN (4-242): 1.38e-39: 150: 14.73
+	Hs
		782203 UB2E1_HUMAN (3-156): 7.23e-67: 203: 33.12		UBA7_HUMAN (17-212): 1.05e-37: 145: 21.50