HitPredict

Interactor 1: MP2K1 (Q02750)
Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1)
Gene: MAP2K1

Interactor 2: AKA12 (Q02952)
A-kinase anchor protein 12 (AKAP-12) (A-kinase anchor protein 250 kDa) (AKAP 250) (Gravin) (Myasthenia gravis autoantigen)
Gene: AKAP12

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	0.60	0.571

Property	Details
Experiments	31980649, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0005925 - focal adhesion GO:0007165 - signal transduction GO:0070374 - positive regulation of ERK1 and ERK2 cascade
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 393 Hs			1 1782
+	Hs
		973758 STK4_HUMAN (37-369): 2.53e-111: 336: 30.06		AKA12_HUMAN (1-1782): 0: 676: 100.00
+	Hs
		414875 CDK1_HUMAN (65-367): 7.64e-110: 325: 26.45		AKA12_HUMAN (1-1782): 0: 676: 100.00
+	Hs
		805396 MP2K3_HUMAN (53-380): 1.39e-65: 214: 36.69		AKA12_HUMAN (1-1782): 0: 676: 100.00
+	Hs
		807692 MP2K1_HUMAN (1-393): 1.26e-125: 369: 100.00		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		734886 MP2K2_HUMAN (1-391): 2.96e-123: 363: 79.40		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		807687 CDK3_HUMAN (65-379): 5.96e-120: 351: 21.81		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		807705 STK4_HUMAN (37-369): 2.53e-111: 336: 30.06		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		807708 STK3_HUMAN (50-369): 2.6e-109: 331: 33.23		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		152597 AKT1_HUMAN (39-389): 1.97e-120: 359: 19.44		KMT2D_HUMAN (47-764): 3.85e-43: 176: 11.48
+	Hs
		153100 MP2K7_HUMAN (35-375): 7.04e-61: 204: 35.49		MAP1B_HUMAN (3-1107): 1.08e-90: 331: 8.57
+	Hs
		734933 MP2K2_HUMAN (1-391): 2.96e-123: 363: 79.40		MDC1_HUMAN (22-1697): 4.38e-13: 77: 12.50
+	Hs
		625528 CDK2_HUMAN (65-367): 2.85e-123: 359: 24.27		TITIN_HUMAN (21-962): 3.34e-13: 77.6: 11.72
+	Hs
		625642 CDK2_HUMAN (65-367): 2.85e-123: 359: 24.27		SRRM2_HUMAN (4-1096): 6.49e-11: 70: 7.25
+	Zm
		17794 A0A1D6NVH8_MAIZE (4-378): 8.39e-109: 325: 32.89		A0A1D6GZI1_MAIZE (15-1778): 2.37e-20: 101: 12.79
+	Hs
		414809 CDK1_HUMAN (65-367): 7.64e-110: 325: 26.45		KI67_HUMAN (1-1782): 2.94e-11: 71.2: 11.31