HitPredict

Interactor 1: TF65 (Q04206)
Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Gene: RELA

Interactor 2: PIAS3 (Q9Y6X2)
E3 SUMO-protein ligase PIAS3 (EC 2.3.2.-) (E3 SUMO-protein transferase PIAS3) (Protein inhibitor of activated STAT protein 3)
Gene: PIAS3

Species: Homo sapiens

Source: innatedb

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.75	0.60	0.672

Property	Details
Experiments	15140884, 22649547, 20547752
Identification Methods	two hybrid, pull down, affinity chromatography technology, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000122 - negative regulation of transcription by RNA polymerase II GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0006357 - regulation of transcription by RNA polymerase II GO:0033234 - negative regulation of protein sumoylation
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 551 Hs			1 628
+	Mm
		222295 TF65_MOUSE (1-551): 0: 577: 87.88		PIAS3_MOUSE (1-628): 0: 867: 96.98
+	Hs
		1152284 Q96CP1_HUMAN (1-226): 4.84e-111: 332: 100.00		Q6IAR4_HUMAN (10-628): 0: 864: 100.00
+	Mm
		638462 NFKB1_MOUSE (7-451): 3.1e-157: 475: 30.19		PIAS2_MOUSE (1-550): 0: 648: 58.48
+	Hs
		978806 TF65_HUMAN (1-551): 0: 601: 100.00		PIAS4_HUMAN (2-477): 2.11e-177: 514: 46.71
+	Mm
		978985 TF65_MOUSE (1-551): 0: 577: 87.88		PIAS4_MOUSE (2-443): 4.93e-173: 503: 50.22
+	Hs
		277867 NFAC1_HUMAN (1-503): 2.8e-93: 307: 18.89		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		1021745 NFAC3_HUMAN (4-522): 1.3e-119: 380: 19.26		ZMIZ1_HUMAN (79-422): 1.82e-84: 287: 24.93