HitPredict

Interactor 1: D6KL2 (Q39183)
Serine/threonine-protein kinase D6PKL2 (EC 2.7.11.1) (D6 protein kinase-like 2) (Serine/threonine-protein kinase AtPK5)
Gene: D6PKL2

Interactor 2: PDPK1 (Q9XF67)
3-phosphoinositide-dependent protein kinase 1 (AtPDK1) (EC 2.7.11.1)
Gene: PDPK1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.68	1.00	0.831

Property	Details
Experiments	16973627, 21798944
Identification Methods	in-gel kinase assay, pull down, two hybrid array
Interaction Type	enzymatic reaction, direct interaction, physical association

Property	Details
Interacting Pfam domains	Pkinase~Pkinase
Common GO terms	GO:0004672 - protein kinase activity GO:0004674 - protein serine/threonine kinase activity GO:0005524 - ATP binding GO:0005737 - cytoplasm GO:0016301 - kinase activity and 1 others more... GO:0106310 - protein serine kinase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 586 At			1 491
+	At
		1247584 Q8RY37_ARATH (1-586): 2.12e-135: 414: 54.52		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1339696 AGC15_ARATH (1-586): 1e-137: 414: 46.89		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		95768 RHS3_ARATH (89-586): 8.62e-139: 414: 51.19		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1352858 D6KL1_ARATH (74-586): 2.39e-138: 413: 69.65		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1322853 D6PK_ARATH (85-586): 8.08e-136: 406: 69.96		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1093333 AGC17_ARATH (50-586): 1.63e-128: 389: 49.02		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1339809 Q9LUK3_ARATH (89-586): 2.5e-128: 387: 58.00		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1352414 KIPK2_ARATH (21-586): 5.26e-123: 387: 48.71		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1340847 Q9M1P3_ARATH (191-537): 1.54e-125: 379: 52.73		PDPK1_ARATH (1-491): 0: 523: 100.00
+	Sl
		1126590 Q5I6E9_SOLLC (1-586): 5.18e-133: 406: 59.52		Q5I6E8_SOLLC (2-491): 1.13e-170: 491: 80.61
+	At
		985500 D6KL3_ARATH (40-586): 3.46e-122: 374: 54.89		PDPK1_ARATH (1-491): 0: 523: 100.00
+	At
		1145519 PID2_ARATH (112-586): 1.57e-112: 347: 42.28		PDPK1_ARATH (1-491): 0: 523: 100.00
+	Dm
		1213430 AKT1_DROME (1-585): 1.7e-127: 389: 14.82		PDPK1_DROME (34-490): 2.03e-149: 448: 30.94
+	Hs
		408561 KPCB_HUMAN (30-585): 5.71e-119: 369: 16.46		AKT1_HUMAN (2-372): 4.75e-146: 428: 31.10
+	Hs
		682081 AKT3_HUMAN (41-585): 1.11e-121: 369: 15.82		AKT1_HUMAN (2-372): 4.75e-146: 428: 31.10
+	Hs
		682218 AKT3_HUMAN (41-585): 1.11e-121: 369: 15.82		AKT2_HUMAN (5-359): 7.83e-146: 428: 30.62
+	Hs
		182071 KPCB_HUMAN (30-585): 5.71e-119: 369: 16.46		PDPK1_HUMAN (18-491): 8.09e-142: 420: 35.39
+	Hs
		182107 KPCI_HUMAN (46-585): 3.38e-107: 336: 20.43		PDPK1_HUMAN (18-491): 8.09e-142: 420: 35.39
+	Sc
		629516 FPK1_YEAST (3-582): 2.42e-122: 384: 27.11		KIN82_YEAST (26-325): 8.77e-60: 210: 38.78
+	Sc
		864555 FPK1_YEAST (3-582): 2.42e-122: 384: 27.11		FPK1_YEAST (26-325): 5e-58: 207: 40.00