HitPredict

Interactor 1: SPSY (Q94BN2)
Spermine synthase (SPMSY) (EC 2.5.1.22) (Spermidine aminopropyltransferase)
Gene: SPMS

Interactor 2: SPDS1 (Q9ZUB3)
Spermidine synthase 1 (SPDSY 1) (EC 2.5.1.16) (Putrescine aminopropyltransferase 1)
Gene: SPDSYN1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.67	1.00	0.821

Property	Details
Experiments	23056524, 12368503
Identification Methods	fluorescent resonance energy transfer, two hybrid, pull down, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Spermine_synt_N~Spermine_synt_N, Spermine_synt_N~Spermine_synth, Spermine_synth~Spermine_synth
Common GO terms	GO:0004766 - spermidine synthase activity GO:0005829 - cytosol GO:0008295 - spermidine biosynthetic process
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 359 At			1 334
+	At
		221283 SPSY_ARATH (1-359): 0: 513: 100.00		SPDS2_ARATH (4-334): 0: 506: 85.12
+	At
		1378384 SPDS1_ARATH (21-357): 3.5e-166: 468: 60.36		SPDS1_ARATH (1-334): 0: 545: 100.00
+	At
		221284 SPDS1_ARATH (21-357): 3.5e-166: 468: 60.36		SPDS2_ARATH (4-334): 0: 506: 85.12
+	At
		221282 SPDS2_ARATH (39-357): 3.76e-164: 463: 61.56		SPDS2_ARATH (4-334): 0: 506: 85.12
+	At
		1277627 SPSY_ARATH (1-359): 0: 513: 100.00		SPSY_ARATH (15-331): 2.36e-153: 436: 60.18
+	Hs
		585949 SPEE_HUMAN (37-353): 3.05e-151: 429: 45.91		SPEE_HUMAN (29-329): 8.77e-158: 445: 49.83
+	Sp
		999866 SPEE_SCHPO (48-359): 8.58e-148: 420: 47.12		SPEE_SCHPO (40-334): 1.1e-153: 434: 51.53
+	Sc
		1014975 SPEE_YEAST (48-353): 4.22e-145: 413: 46.08		SPSY_YEAST (38-329): 7.42e-137: 392: 42.33
+	Ec
		448097 SPEE_ECOLI (87-358): 2.62e-119: 348: 34.80		SPEE_ECOLI (44-334): 2.03e-122: 355: 36.61