HitPredict

Interactor 1: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Interactor 2: NFIP1 (Q9BT67)
NEDD4 family-interacting protein 1 (Breast cancer-associated protein SGA-1M) (NEDD4 WW domain-binding protein 5) (Putative MAPK-activating protein PM13) (Putative NF-kappa-B-activating protein 164) (Putative NFKB and MAPK-activating protein)
Gene: NDFIP1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.70	0.60	0.651

Property	Details
Experiments	25632008, 26245901, 28747490
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005938 - cell cortex GO:0006511 - ubiquitin-dependent protein catabolic process GO:0035739 - CD4-positive, alpha-beta T cell proliferation GO:0045732 - positive regulation of protein catabolic process GO:2000562 - negative regulation of CD4-positive, alpha-beta T cell proliferation
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 903 Hs			1 221
+	Mm
		1208047 ITCH_MOUSE (1-903): 0: 885: 91.16		NFIP1_MOUSE (1-221): 1.54e-136: 383: 97.29
+	Hs
		1294871 ITCH_HUMAN (1-903): 0: 926: 100.00		NFIP2_HUMAN (12-220): 5.72e-98: 290: 55.46
+	Hs
		1311857 WWP1_HUMAN (1-903): 0: 832: 57.54		NFIP1_HUMAN (1-221): 1.9e-136: 383: 100.00
+	Hs
		125686 WWP2_HUMAN (1-903): 0: 816: 54.08		NFIP1_HUMAN (1-221): 1.9e-136: 383: 100.00
+	Hs
		1299617 NED4L_HUMAN (5-900): 0: 762: 32.14		NFIP1_HUMAN (1-221): 1.9e-136: 383: 100.00
+	Hs
		1327920 WWP1_HUMAN (1-903): 0: 832: 57.54		NFIP2_HUMAN (12-220): 5.72e-98: 290: 55.46
+	Hs
		125746 WWP2_HUMAN (1-903): 0: 816: 54.08		NFIP2_HUMAN (12-220): 5.72e-98: 290: 55.46
+	Hs
		808249 NEDD4_HUMAN (90-900): 0: 673: 34.16		NFIP1_HUMAN (1-221): 1.9e-136: 383: 100.00
+	Hs
		1299647 NED4L_HUMAN (5-900): 0: 762: 32.14		NFIP2_HUMAN (12-220): 5.72e-98: 290: 55.46
+	Mm
		93279 E9PXB7_MOUSE (86-900): 0: 654: 34.22		NFIP1_MOUSE (1-221): 1.54e-136: 383: 97.29
+	Hs
		808291 NEDD4_HUMAN (90-900): 0: 673: 34.16		NFIP2_HUMAN (12-220): 5.72e-98: 290: 55.46
+	Dm
		1374794 SUDX_DROME (19-903): 0: 788: 48.29		Q9VVJ5_DROME (100-221): 4.9e-53: 173: 40.98
+	Dm
		1374786 NEDD4_DROME (3-901): 0: 720: 29.95		Q9VVJ5_DROME (100-221): 4.9e-53: 173: 40.98
+	Rn
		1137274 NEDD4_RAT (7-426): 3.03e-54: 205: 18.07		NFIP1_RAT (1-221): 2.44e-135: 380: 97.74
+	Mm
		808368 NEDD4_MOUSE (13-426): 2.27e-52: 199: 18.38		NFIP1_MOUSE (1-221): 1.54e-136: 383: 97.29
+	Hs
		1311860 SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71		NFIP1_HUMAN (1-221): 1.9e-136: 383: 100.00
+	Dm
		1360300 SMUF1_DROME (16-518): 1.15e-39: 161: 16.19		Q9VVJ5_DROME (100-221): 4.9e-53: 173: 40.98