HitPredict

Interactor 1: SMAD5 (Q99717)
Mothers against decapentaplegic homolog 5 (MAD homolog 5) (Mothers against DPP homolog 5) (JV5-1) (SMAD family member 5) (SMAD 5) (Smad5) (hSmad5)
Gene: SMAD5

Interactor 2: SMUF2 (Q9HAU4)
E3 ubiquitin-protein ligase SMURF2 (hSMURF2) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF2) (SMAD ubiquitination regulatory factor 2) (SMAD-specific E3 ubiquitin-protein ligase 2)
Gene: SMURF2

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	0.60	0.719

Property	Details
Experiments	15221015, 28514442, 33961781, 20484049, 15231748
Identification Methods	affinity chromatography technology, enzymatic study, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 748
+	Mm
		56826 SMAD1_MOUSE (3-465): 0: 638: 89.89		SMUF2_MOUSE (1-748): 0: 835: 99.20
+	Hs
		1078760 SMAD1_HUMAN (3-465): 0: 637: 89.68		SMUF2_HUMAN (1-748): 0: 834: 100.00
+	Mm
		936618 SMAD1_MOUSE (3-465): 0: 638: 89.89		SMUF1_MOUSE (1-748): 0: 761: 73.01
+	Hs
		1305888 SMAD5_HUMAN (1-465): 0: 650: 100.00		SMUF1_HUMAN (1-748): 0: 748: 70.44
+	Hs
		1078761 SMAD1_HUMAN (3-465): 0: 637: 89.68		SMUF1_HUMAN (1-748): 0: 748: 70.44
+	Hs
		950287 SMAD3_HUMAN (4-465): 0: 550: 58.97		SMUF2_HUMAN (1-748): 0: 834: 100.00
+	Xl
		945613 P79947_XENLA (3-465): 0: 643: 89.87		SMUF1_XENLA (1-748): 0: 739: 70.96
+	Hs
		950291 SMAD3_HUMAN (4-465): 0: 550: 58.97		SMUF1_HUMAN (1-748): 0: 748: 70.44
+	Hs
		808240 SMAD5_HUMAN (1-465): 0: 650: 100.00		NEDD4_HUMAN (104-746): 0: 628: 38.73
+	Hs
		808135 SMAD1_HUMAN (3-465): 0: 637: 89.68		NEDD4_HUMAN (104-746): 0: 628: 38.73
+	Hs
		808087 SMAD3_HUMAN (4-465): 0: 550: 58.97		NEDD4_HUMAN (104-746): 0: 628: 38.73
+	Hs
		1305881 SMAD5_HUMAN (1-465): 0: 650: 100.00		WWP1_HUMAN (2-395): 4.38e-20: 97.5: 15.47
+	Hs
		1305892 SMAD5_HUMAN (1-465): 0: 650: 100.00		HECW2_HUMAN (19-281): 8.01e-17: 87.5: 14.80
+	Hs
		1078757 SMAD1_HUMAN (3-465): 0: 637: 89.68		WWP1_HUMAN (2-395): 4.38e-20: 97.5: 15.47
+	Dm
		785477 MAD_DROME (1-465): 0: 596: 72.47		SMUF1_DROME (7-225): 1.83e-30: 131: 48.70
+	Dm
		785516 MAD_DROME (1-465): 0: 596: 72.47		SUDX_DROME (10-428): 3.95e-22: 104: 14.41
+	Hs
		950249 SMAD3_HUMAN (4-465): 0: 550: 58.97		NED4L_HUMAN (5-396): 1.96e-36: 149: 18.58
+	Hs
		125369 SMAD3_HUMAN (4-465): 0: 550: 58.97		WWP2_HUMAN (1-416): 7.64e-26: 116: 17.39
+	Hs
		950248 SMAD3_HUMAN (4-465): 0: 550: 58.97		ITCH_HUMAN (1-426): 2.45e-23: 107: 16.44
+	Hs
		950278 SMAD3_HUMAN (4-465): 0: 550: 58.97		WWP1_HUMAN (2-395): 4.38e-20: 97.5: 15.47