HitPredict

Interactor 1: BRK1 (Q8WUW1)
Protein BRICK1 (BRK1)
Gene: BRK1

Interactor 2: WASF3 (Q9UPY6)
Actin-binding protein WASF3 (Protein WAVE-3) (Verprolin homology domain-containing protein 3) (Wiskott-Aldrich syndrome protein family member 3) (WASP family protein member 3)
Gene: WASF3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.73	0.60	0.662

Property	Details
Experiments	28514442, 33961781, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005856 - cytoskeleton GO:0030027 - lamellipodium GO:0031209 - SCAR complex GO:0070062 - extracellular exosome GO:2000601 - positive regulation of Arp2/3 complex-mediated actin nucleation
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 75 Hs			1 502
+	Mm
		1296532 BRK1_MOUSE (1-75): 4.3e-36: 117: 98.67		WASF2_MOUSE (1-484): 9.93e-66: 222: 42.56
+	Hs
		1357188 BRK1_HUMAN (1-75): 1.6e-36: 119: 100.00		WASF1_HUMAN (1-500): 3.44e-59: 206: 43.57
+	Bt
		72267 A8YXX8_BOVIN (1-75): 1.29e-36: 119: 98.67		WASF1_BOVIN (1-500): 9.36e-59: 205: 43.93
+	Hs
		1357241 BRK1_HUMAN (1-75): 1.6e-36: 119: 100.00		WASF2_HUMAN (1-484): 2.02e-57: 200: 43.60
+	Dm
		120194 Q8MLQ0_DROME (2-75): 7.17e-36: 117: 72.97		M9PCP4_DROME (1-317): 1.62e-48: 178: 37.90
+	Dm
		99167 Q8MLQ0_DROME (2-75): 7.17e-36: 117: 72.97		F2FB81_DROME (1-209): 1.44e-42: 162: 54.55
+	At
		1231032 BRK1_ARATH (12-69): 7.32e-24: 87: 43.10		SCAR1_ARATH (1-271): 7.2e-51: 187: 18.49
+	At
		1376670 BRK1_ARATH (12-69): 7.32e-24: 87: 43.10		SCAR3_ARATH (1-162): 6.65e-47: 177: 21.69
+	At
		1220926 BRK1_ARATH (12-69): 7.32e-24: 87: 43.10		SCAR2_ARATH (1-194): 3.95e-37: 148: 21.36
+	Dd
		1200683 BRK1_DICDI (9-66): 2.51e-19: 75.1: 48.28		SCAR_DICDI (23-501): 1.21e-28: 119: 22.04