HitPredict

Interactor 1: RPB7 (O14459)
DNA-directed RNA polymerase II subunit rpb7 (RNA polymerase II subunit B7)
Gene: RPB7

Interactor 2: RPB1 (P36594)
DNA-directed RNA polymerase II subunit rpb1 (RNA polymerase II subunit 1) (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit)
Gene: RPB1

Species: Schizosaccharomyces pombe 972h-

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.933

Property	Details
Experiments	10648788, 9325316, 15743411, 9642054, 16204182, 14534314
Identification Methods	pull down, affinity chromatography technology, far western blotting, anti bait coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	RNA_pol_Rpb1_1~SHS2_Rpb7-N, RNA_pol_Rpb1_2~SHS2_Rpb7-N
Common GO terms	GO:0005634 - nucleus GO:0005665 - RNA polymerase II, core complex GO:0005829 - cytosol GO:0006367 - transcription initiation at RNA polymerase II promoter GO:0016591 - RNA polymerase II, holoenzyme
Homologous interactions	12

Homologous interactions

Color coding for hit scores

	1 172 Sp			1 1752
+	Sc
		375342 RPB7_YEAST (3-172): 2.83e-65: 199: 52.94		RPB1_YEAST (1-1564): 0: 1617: 61.06
+	Hs
		156013 RPB7_HUMAN (3-172): 7.42e-65: 197: 49.42		RPC1_HUMAN (1-1460): 0: 1302: 33.85
+	Hs
		156086 RPC8_HUMAN (3-172): 2.89e-46: 152: 18.72		RPC1_HUMAN (1-1460): 0: 1302: 33.85
+	Sc
		375597 RPC8_YEAST (3-149): 4.1e-44: 146: 20.00		RPC1_YEAST (9-1465): 0: 1301: 32.72
+	Hs
		317099 RPB7_HUMAN (3-172): 7.42e-65: 197: 49.42		RPA1_HUMAN (919-1452): 1.39e-40: 167: 14.82
+	At
		64324 NRPE7_ARATH (3-172): 2.78e-56: 176: 23.86		NRPE1_ARATH (843-1455): 6e-34: 145: 14.90
+	Hs
		317192 RPC8_HUMAN (3-172): 2.89e-46: 152: 18.72		RPA1_HUMAN (919-1452): 1.39e-40: 167: 14.82
+	At
		64326 NRPE7_ARATH (3-172): 2.78e-56: 176: 23.86		NRPD1_ARATH (844-1491): 4.34e-33: 142: 15.89
+	At
		1227791 NRPD7_ARATH (3-171): 1.52e-54: 172: 24.71		NRPD1_ARATH (844-1491): 4.34e-33: 142: 15.89
+	Bt
		102298 RPB7_BOVIN (3-172): 7.42e-65: 197: 49.42		RPB1_BOVIN (1489-1699): 7.68e-16: 86.1: 52.56
+	Hs
		624849 RPB7_HUMAN (3-172): 7.42e-65: 197: 49.42		RPB1_HUMAN (1489-1699): 7.68e-16: 86.1: 52.56
+	Hs
		625110 RPC8_HUMAN (3-172): 2.89e-46: 152: 18.72		RPB1_HUMAN (1489-1699): 7.68e-16: 86.1: 52.56