HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: ECHP (Q08426)
Peroxisomal bifunctional enzyme (PBE) (PBFE) (L-bifunctional protein) (LBP) (Multifunctional enzyme 1) (MFE1) [Includes: Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase (EC 4.2.1.17) (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]
Gene: EHHADH

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.71	0.60	0.654

Property	Details
Experiments	31515488, unassigned1304, 25416956
Identification Methods	two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0019899 - enzyme binding
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 667 Hs			1 723
+	Hs
		995604 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		995562 TRI63_HUMAN (1-380): 2.91e-86: 276: 24.09		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		995558 TRI41_HUMAN (1-655): 3.11e-77: 260: 13.81		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		995583 TRI54_HUMAN (1-380): 7.45e-80: 259: 22.80		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		778193 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		ECHA_HUMAN (6-713): 0: 749: 27.92
+	Hs
		536801 TRI27_HUMAN (90-433): 1.46e-30: 128: 14.08		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		995585 TRIM5_HUMAN (92-376): 2.38e-28: 121: 17.24		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		778144 TRI14_HUMAN (152-652): 3.33e-73: 244: 18.90		ECHA_HUMAN (6-713): 0: 749: 27.92
+	Hs
		734605 TRI23_HUMAN (8-252): 7.45e-19: 92.4: 25.88		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		584937 RO52_HUMAN (1-295): 2.16e-18: 90.5: 12.70		ECHP_HUMAN (1-723): 0: 880: 100.00
+	Hs
		1088161 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		HCDH_HUMAN (297-583): 2.62e-110: 338: 30.64
+	Hs
		1156934 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		HIBCH_HUMAN (5-343): 1.53e-96: 305: 18.91
+	Hs
		1172352 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		CRYL1_HUMAN (300-566): 8.53e-87: 277: 22.74
+	Hs
		671469 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		ECHM_HUMAN (1-245): 2.08e-79: 257: 28.80
+	Hs
		1022526 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		ECH1_HUMAN (5-243): 2.18e-70: 234: 24.32
+	Hs
		671486 TRI63_HUMAN (1-380): 2.91e-86: 276: 24.09		ECHM_HUMAN (1-245): 2.08e-79: 257: 28.80
+	Hs
		1022559 SPRY4_HUMAN (486-660): 1.38e-18: 86.7: 28.65		ECH1_HUMAN (5-243): 2.18e-70: 234: 24.32