HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: PAK2 (Q13177)
Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (PAK65) (S6/H4 kinase) (p21-activated kinase 2) (PAK-2) (p58) [Cleaved into: PAK-2p27 (p27); PAK-2p34 (p34) (C-t-PAK2)]
Gene: PAK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.90	0.60	0.736

Property	Details
Experiments	26186194, 28514442, 32296183, 33961781, 35271311, unassigned1304
Identification Methods	affinity chromatography technology, two hybrid, two hybrid prey pooling approach, two hybrid array, anti tag coimmunoprecipitation, validated two hybrid
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0014069 - postsynaptic density GO:0060996 - dendritic spine development
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 524
+	Hs
		557677 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		PAK2_HUMAN (1-524): 0: 538: 100.00
+	Hs
		209044 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		PAK1_HUMAN (1-523): 6.81e-169: 490: 74.63
+	Hs
		557676 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		PAK1_HUMAN (1-523): 6.81e-169: 490: 74.63
+	Mm
		292046 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		PAK1_MOUSE (1-523): 1.84e-167: 486: 73.35
+	Hs
		277595 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		PAK3_HUMAN (1-523): 8.9e-162: 472: 71.71
+	Ce
		1089181 Q95PW9_CAEEL (1-379): 5.2e-75: 239: 37.88		PK1_CAEEL (9-522): 1.29e-137: 411: 52.21
+	Hs
		412169 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		PAK2_HUMAN (1-524): 0: 538: 100.00
+	Hs
		442822 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		PAK2_HUMAN (1-524): 0: 538: 100.00
+	Hs
		681710 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		AKT1_HUMAN (141-523): 1.99e-133: 397: 21.83
+	Mm
		643056 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		AKT1_MOUSE (141-523): 6.96e-134: 398: 21.83
+	Hs
		655233 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		AKT1_HUMAN (141-523): 1.99e-133: 397: 21.83
+	Hs
		412046 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		AKT1_HUMAN (141-523): 1.99e-133: 397: 21.83
+	Mm
		403418 SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33		AKT1_MOUSE (141-523): 6.96e-134: 398: 21.83
+	Hs
		568554 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		KAPCA_HUMAN (207-520): 9.62e-131: 385: 22.91
+	Hs
		411994 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		KAPCA_HUMAN (207-520): 9.62e-131: 385: 22.91
+	Hs
		412014 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		KAPCB_HUMAN (207-524): 1.39e-130: 385: 22.94
+	Hs
		129195 P85B_HUMAN (21-380): 7.78e-23: 102: 13.10		KAPCA_HUMAN (207-520): 9.62e-131: 385: 22.91
+	Hs
		412163 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		STK4_HUMAN (221-518): 9.32e-125: 375: 40.00
+	Dm
		1214366 NCK_DROME (6-377): 1.75e-68: 226: 39.90		PAK_DROME (8-177): 3.93e-22: 102: 36.96
+	Hs
		412388 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		PAK5_HUMAN (67-179): 3.87e-12: 70.7: 25.64