HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: DOK1 (Q99704)
Docking protein 1 (Downstream of tyrosine kinase 1) (p62(dok)) (pp62)
Gene: DOK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.899

Property	Details
Experiments	26186194, 28514442, 9737977, 33961781
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PH~SH3_1, PH~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 481
+	Mm
		223011 NCK2_MOUSE (1-380): 3.58e-98: 298: 96.05		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Hs
		557780 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		DOK1_HUMAN (1-481): 0: 564: 100.00
+	Mm
		956524 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Hs
		655452 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		DOK1_HUMAN (1-481): 0: 564: 100.00
+	Hs
		454844 FGR_HUMAN (7-155): 1.02e-23: 104: 18.54		DOK1_HUMAN (1-481): 0: 564: 100.00
+	Mm
		643076 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Mm
		223005 NCK2_MOUSE (1-380): 3.58e-98: 298: 96.05		DOK2_MOUSE (2-426): 3.12e-118: 354: 31.31
+	Hs
		208960 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		DOK2_HUMAN (2-426): 3.33e-116: 349: 34.09
+	Mm
		250957 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		DOK2_MOUSE (2-426): 3.12e-118: 354: 31.31
+	Hs
		235041 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		DOK2_HUMAN (2-426): 3.33e-116: 349: 34.09
+	Hs
		208999 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		IRS1_HUMAN (6-373): 1.95e-76: 262: 16.33
+	Hs
		235051 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		DOK2_HUMAN (2-426): 3.33e-116: 349: 34.09
+	Hs
		151716 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		IRS4_HUMAN (6-404): 5.55e-95: 314: 14.35
+	Rn
		724634 P85A_RAT (163-379): 8.5e-24: 105: 16.14		IRS1_RAT (6-373): 3.69e-78: 267: 17.27
+	Mm
		643063 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		IRS1_MOUSE (6-373): 1.1e-77: 265: 17.27
+	Hs
		655240 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		IRS1_HUMAN (6-373): 1.95e-76: 262: 16.33
+	Mm
		643075 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		IRS2_MOUSE (6-371): 1.55e-70: 245: 15.10
+	Rn
		94391 P85A_RAT (163-379): 8.5e-24: 105: 16.14		F1MAL5_RAT (6-371): 8.44e-69: 240: 15.10
+	Hs
		655516 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		IRS2_HUMAN (6-384): 1.12e-67: 237: 14.05
+	Rn
		139726 P85A_RAT (163-379): 8.5e-24: 105: 16.14		O08724_RAT (7-315): 2.1e-60: 207: 19.09