HitPredict

Interactor 1: PAK3 (O75914)
Serine/threonine-protein kinase PAK 3 (EC 2.7.11.1) (Beta-PAK) (Oligophrenin-3) (p21-activated kinase 3) (PAK-3)
Gene: PAK3

Interactor 2: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.71	1.00	0.843

Property	Details
Experiments	25301945, 11478794, 11862216
Identification Methods	affinity chromatography technology, two hybrid, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	PBD~Ras, Pkinase~Ras
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0032956 - regulation of actin cytoskeleton organization GO:0035556 - intracellular signal transduction and 1 others more... GO:0098978 - glutamatergic synapse
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 559 Hs			1 192
+	Hs
		277619 PAK3_HUMAN (1-559): 0: 561: 100.00		RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64
+	Hs
		277599 PAK3_HUMAN (1-559): 0: 561: 100.00		CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31
+	Hs
		925244 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00
+	Mm
		103566 G5E884_MOUSE (1-557): 1.32e-170: 496: 76.86		RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00
+	Hs
		564505 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67
+	Mm
		103575 G5E884_MOUSE (1-557): 1.32e-170: 496: 76.86		RHOQ_MOUSE (4-187): 8.33e-85: 250: 64.13
+	Mm
		292040 PAK1_MOUSE (1-557): 1.07e-168: 491: 75.44		RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00
+	Mm
		103567 G5E884_MOUSE (1-557): 1.32e-170: 496: 76.86		RAC2_MOUSE (1-192): 6.16e-83: 245: 91.67
+	Hs
		545350 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19
+	Hs
		1026226 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64
+	Mm
		292041 PAK1_MOUSE (1-557): 1.07e-168: 491: 75.44		RAC2_MOUSE (1-192): 6.16e-83: 245: 91.67
+	Mm
		925809 PAK2_MOUSE (1-558): 7.87e-166: 483: 71.58		RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00
+	Hs
		892345 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19
+	Hs
		950963 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35
+	Rn
		723755 PAK1_RAT (1-557): 1.66e-171: 498: 77.03		CDC42_RAT (1-192): 1.4e-78: 234: 70.31
+	Hs
		893957 PAK1_HUMAN (1-557): 6.04e-171: 497: 76.86		CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31
+	Mm
		103565 G5E884_MOUSE (1-557): 1.32e-170: 496: 76.86		CDC42_MOUSE (1-192): 1.4e-78: 234: 70.31
+	Mm
		292039 PAK1_MOUSE (1-557): 1.07e-168: 491: 75.44		CDC42_MOUSE (1-192): 1.4e-78: 234: 70.31
+	Mm
		292048 PAK1_MOUSE (1-557): 1.07e-168: 491: 75.44		RHOA_MOUSE (5-192): 1.43e-77: 231: 57.98
+	Hs
		436282 PAK2_HUMAN (1-558): 2.03e-165: 482: 71.58		RHOC_HUMAN (5-192): 8.68e-75: 224: 57.45